Protein Info for Psest_2151 in Pseudomonas stutzeri RCH2

Annotation: formate dehydrogenase, alpha subunit, proteobacterial-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1021 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 5 to 1020 (1016 residues), 1477.3 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 46 to 105 (60 residues), 58.1 bits, see alignment 1e-19 PF00384: Molybdopterin" amino acids 109 to 592 (484 residues), 84.1 bits, see alignment E=1.5e-27 amino acids 616 to 657 (42 residues), 24.5 bits, see alignment (E = 2e-09) PF01568: Molydop_binding" amino acids 898 to 1014 (117 residues), 47.2 bits, see alignment E=3.1e-16

Best Hits

Swiss-Prot: 61% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 62% identity to bpl:BURPS1106A_A2259)

MetaCyc: 60% identical to formate dehydrogenase N subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMS1 at UniProt or InterPro

Protein Sequence (1021 amino acids)

>Psest_2151 formate dehydrogenase, alpha subunit, proteobacterial-type (Pseudomonas stutzeri RCH2)
MTVRVSRRQFLKFGAAGLGASSMAMMGFAPEEALASVRHFKLTGAKVTRNICTYCSVGCG
MLLYARGDGAINSHDHIFHVEGDPDHPVSRGSLCPRGAGVLDFIKSDSRVRYPQVREPGT
DEWKRISWDEALDRIARLMKDDRDRNFETHDDEGQLVNRWMTTSMVAASACTNETAYLTQ
KITRALGILKLDNQARVUHGPTVAGLAPSFGRGAMTNSWVDIRNANIILSMGGNSAEAHP
VGFRWVMQAKERSDAILISIDPRYNRTTAVADYHAWIRTGTDIVFLGGLISFLIENDRYA
HEYVLHYTDAAHLVREGFEFNDGLFTGYDEERHQYDKATWNFDLDEQGFVRRDETLQHPR
CVFQMMRKHYSRYTVEMVERVCGMPQAKVLQLWELLAQSAAPDKTMTILYALGWTQHSIG
AQMIRTGAMVQLLLGNMGMPGGGVNALRGHSNIQGLTDIGLLSNLLTGYMTLPAAGAQNY
DDYIKQKIRQPLLPNQVSYWKHYERFFVSMMKAFYGDAARAENNWCYDWLPKLSEPLYDV
LYAVNDMTKGKMTGAFCQGFNILAAFPNKAKVLDGLSKLKWLVIMDPLATETGEFWKNHG
EFNDVDPSQIQTAVFRLPTTTFAEDDGSITNSSRWLQWHHQAAPPPGEAKTDTAILAGVF
HRLQKLYREEGGAFPEPILNLTWNYSDPAHPAAAELAQEYNGRALADIEDQGRIIRRKGE
LLNDFGELRADGSTSCGCWIYSGSWTEQGNMMDRRNNSDPYDIGVTLGWAWAWPLNRRIL
YNRASARPDGTPWDPNRALVWWNGERWVGADVPDYPIAAPPEDDVKPFILTESGTGHFFA
SEWLGEGPFPEHYEPLESPIPNNPLHPNNPLAYHNPVARIFPEDRDTFGTFEEFPYAATT
YRLTEHFHYWTTHVLLNAIVQPEKFVEIGEVLAGELGIAAGEKVRVRSKRGHIDAVAVVT
KRLRPLQVDGRTVHQVGIPIHWGFTGVARKAHLTNTLTPFVGDGNTQTPEFKSFLVNVEK
L