Protein Info for Psest_2148 in Pseudomonas stutzeri RCH2

Annotation: Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 42 to 232 (191 residues), 178.3 bits, E=2.6e-56
• PF08659: KR
  • amino acids 43 to 204 (162 residues), 51.9 bits, E=1.8e-17
• PF01370: Epimerase
  • amino acids 45 to 182 (138 residues), 23 bits, E=1e-08
• PF13561: adh_short_C2
  • amino acids 50 to 280 (231 residues), 221.8 bits, E=2e-69

Best Hits

Swiss-Prot: 63% identical to YHXC_BACSU: Uncharacterized oxidoreductase YhxC (yhxC) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 86% identity to psa:PST_2163)

MetaCyc: 50% identical to aldehyde reductase (chloroplastic) (Arabidopsis thaliana col)
Aldehyde reductase. [EC: 1.1.1.21]; Alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.2 [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.- [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.- [EC: 1.1.1.21, 1.1.1.2]

Predicted SEED Role

"putative short-chain dehydrogenase"

MetaCyc Pathways

• methylglyoxal degradation III (1/2 steps found)
• glycerol degradation to butanol (10/16 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (1/4 steps found)
• superpathway of methylglyoxal degradation (3/8 steps found)
• detoxification of reactive carbonyls in chloroplasts (4/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• traumatin and (Z)-3-hexen-1-yl acetate biosynthesis (3/12 steps found)
• superpathway of lipoxygenase (4/22 steps found)
• superpathway of pentose and pentitol degradation (7/42 steps found)

KEGG Metabolic Maps

• Caprolactam degradation
• Fructose and mannose metabolism
• Galactose metabolism
• Glycerolipid metabolism
• Glycolysis / Gluconeogenesis
• Pentose and glucuronate interconversions
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.2 or 1.1.1.21

Sequence Analysis Tools

See L0GLM6 at UniProt or InterPro

Protein Sequence (285 amino acids)

>Psest_2148 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) (Pseudomonas stutzeri RCH2)
MAEDNQTLPPQEQPEPGKEGLMNPRPEYRGEDYKAAGKLEGKTAIITGGDSGIGRSVAVL
FAREGADVAILYLDQHQDAEETRTVVEQYGRRCLTFAGDVADRDVCRKVIDETLAAFGKL
DILVNNAAEQHPQEKLEDISEEQWEKTFRTNIFGMFQMTKAVLPHLGKGASIINTTSVTA
YKGSPQLLDYSATKGAITAFTRSLSMNLAERGIRVNGVAPGPIWTPLISSTFDADEVAEF
GSNTPMKRPGQPDEVAPAYVYLASSDAAYVSGQVIHVNGGTVVNG