Protein Info for HP15_2059 in Marinobacter adhaerens HP15

Annotation: UDP-N-acetylenolpyruvoylglucosamine reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00179: UDP-N-acetylenolpyruvoylglucosamine reductase
  • amino acids 15 to 339 (325 residues), 230.4 bits, E=1.4e-72
• PF01565: FAD_binding_4
  • amino acids 26 to 158 (133 residues), 77.6 bits, E=8.1e-26
• PF02873: MurB_C
  • amino acids 218 to 340 (123 residues), 96.5 bits, E=9.2e-32

Best Hits

Swiss-Prot: 46% identical to MURB_PSEPF: UDP-N-acetylenolpyruvoylglucosamine reductase (murB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00075, UDP-N-acetylmuramate dehydrogenase [EC: 1.1.1.158] (inferred from 67% identity to maq:Maqu_1736)

Predicted SEED Role

"UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)" in subsystem Peptidoglycan Biosynthesis or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 1.1.1.158)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
• peptidoglycan recycling I (8/14 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.158

Sequence Analysis Tools

See E4PRB4 at UniProt or InterPro

Protein Sequence (348 amino acids)

>HP15_2059 UDP-N-acetylenolpyruvoylglucosamine reductase (Marinobacter adhaerens HP15)
MSQQPEIRENIELEALNTLHVPAKARFYVEVHTSDELVRSLDWASSEDQEVLILGGGSNL
VFAGDFAGLVVRLAIRGRRWEHIEGHRATLVLGAGENWHEAVLYAARAGYRGIENLALIP
GTAGAAPVQNIGAYGVELGDSLESVTALDRNSGELVVLDNAQCRFSYRDSLFKQTPGRYV
ITEIRLGLSRSRALVLGYRDLREYLGDTAETALDPLHVAEAVMAIRRRKLPDPDMIPNAG
SFFKNPVVDGETFEVLSERHPDIVAYPQEQGVKLAAAWLIDQSGWKGFRNARVGVHNRQA
LVLINHSGGTGADILELAREVRQSVVDRFGVTLEMEPGIVGGSGRENP