Protein Info for Psest_2144 in Pseudomonas stutzeri RCH2

Annotation: The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1056 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 342 to 362 (21 residues), see Phobius details amino acids 369 to 390 (22 residues), see Phobius details amino acids 396 to 419 (24 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details amino acids 472 to 498 (27 residues), see Phobius details amino acids 548 to 566 (19 residues), see Phobius details amino acids 877 to 899 (23 residues), see Phobius details amino acids 906 to 928 (23 residues), see Phobius details amino acids 934 to 958 (25 residues), see Phobius details amino acids 979 to 1000 (22 residues), see Phobius details amino acids 1011 to 1037 (27 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 3 to 1052 (1050 residues), 1199.3 bits, see alignment E=0 PF00873: ACR_tran" amino acids 3 to 1038 (1036 residues), 1276.4 bits, see alignment E=0 PF02355: SecD_SecF_C" amino acids 343 to 498 (156 residues), 25.6 bits, see alignment E=1.1e-09 PF03176: MMPL" amino acids 347 to 501 (155 residues), 43.5 bits, see alignment E=3.3e-15

Best Hits

KEGG orthology group: None (inferred from 88% identity to avn:Avin_33870)

Predicted SEED Role

"Multidrug efflux transporter MexF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIW4 at UniProt or InterPro

Protein Sequence (1056 amino acids)

>Psest_2144 The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family (Pseudomonas stutzeri RCH2)
MNFSQFFIRRPIFAAVLSLVILIGGAISLFQLPISEYPEVVPPTVVVRANFPGANPKVIG
ETVASPLEQAITGVEGMLYMSSQATADGKLTLTITFGLGTDLDNAQVQVQNRVTRTMPTL
PTEVQRLGVTVDKASPDLTMVVHLTSPDQRYDMLYLSNYAALNVKDELARLDGIGDVQLF
GMGDYSLRVWLDPEKVASRNLTASDVVNAIREQNRQVAAGSLGAPPAPGATDFQLSINTQ
GRLVTEEEFENIIIRAGEDGSITRLRDIARVELGSSQYALRSLLNNQPAVAIPVFQRPGS
NAIEISDSVRARMAELKRDFPEGVDYEIVYDPTIFVRGSIEAVVHTLLEAIVLVVLVVIL
FLQTWRASIIPLAAVPVSLIGTFAVMHLLGFSLNALSLFGLVLAIGIVVDDAIVVVENVE
RNIGLGKSPEEATRQAMKEVTGPIIATALVLCAVFIPTAFISGLTGQFYQQFALTIAIST
VISAFNSLTLSPALAAALLRSHDAPKDGFSRLLDRIFGGWLFAPFNRMFDRASHGYVGLV
RRILRGSGIALVVYVGLVGLGYMGFASTPTGFVPPQDKQYLVAFAQLPDAATLDRTEDVI
KRMSEIAGKHPGVENTVAFPGLSINGFTNSPNSGIVFTPLKPFDERKDPSLSANAIAADL
NGQFAQIQDAFIAIFPPPPVQGLGTIGGFRVQVQDRGNLGYEELYSQVQNVIAKSADYPE
LAGLFTSYQVNVPQVDADIDREKAKTHGVPIDEIFDTMQVYLGSLYANDFNRFGRTYQVN
VQADQKFRLAPEQIGQLKVRNNRGEMVPLSTFVNVTDSAGPDRVMHYNGFLTAEINGAAA
PGYSSGQAEAAMERLLKAELPNGMSYEWTELTYQQILAGNTAIFVFPLCVLLAFLVLAAQ
YESWSLPLAVILIVPMTLLSAITGVILAGSDNNVFTQIGLIVLVGLACKNAILIVEFAKD
KQEEGMDRLAAILEACRLRLRPILMTSFAFIMGVVPLVLSSGAGAEMRHAMGVAVFSGML
GVTFFGLLLTPVFYLVIRAFVEKREARKAVYKEVQA