Protein Info for Psest_2139 in Pseudomonas stutzeri RCH2

Annotation: Cation/multidrug efflux pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1026 transmembrane" amino acids 9 to 31 (23 residues), see Phobius details amino acids 336 to 353 (18 residues), see Phobius details amino acids 360 to 384 (25 residues), see Phobius details amino acids 390 to 410 (21 residues), see Phobius details amino acids 431 to 451 (21 residues), see Phobius details amino acids 463 to 483 (21 residues), see Phobius details amino acids 522 to 542 (21 residues), see Phobius details amino acids 848 to 870 (23 residues), see Phobius details amino acids 877 to 896 (20 residues), see Phobius details amino acids 902 to 924 (23 residues), see Phobius details amino acids 951 to 970 (20 residues), see Phobius details amino acids 982 to 1007 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1007 (1005 residues), 1122.2 bits, see alignment E=0

Best Hits

Swiss-Prot: 64% identical to MDTB_ESCF3: Multidrug resistance protein MdtB (mdtB) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)

KEGG orthology group: K07788, RND superfamily, multidrug transport protein MdtB (inferred from 82% identity to avn:Avin_28810)

MetaCyc: 64% identical to multidrug efflux pump RND permease subunit MdtB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-353; TRANS-RXN-92

Predicted SEED Role

"Acriflavin resistance plasma membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIV9 at UniProt or InterPro

Protein Sequence (1026 amino acids)

>Psest_2139 Cation/multidrug efflux pump (Pseudomonas stutzeri RCH2)
MNISRLFILRPVATTLSMVAILLAGLIAYKLLPVAALPQVDYPTIRVMTLYPGASPEVMT
SAVTAPLERQFGQMPGLAQMSSTSSGGASVITLRFSLDVALDVAEQEVQAAINGANNLLP
NDLPAPPVYNKVNPADTPVLTLAVTSESLPLPKLHDLVDTRMAQKLAQINGVGMVSIAGG
QRPAVRIRTNPEALAAYGLSLADVRSLITSSNVNQPKGNFDGPTRVSMLDANDQLKTPEE
YAELILTYQDGAALRLKDVADIVDGAENERLAAWANESQAVLLNVQRQPGANVIDVVERI
QALLPEVTASMPAGLDVVVLTDRTQTIRAAVTDVQHELMLATFLVVMVTFVFLKKLSATV
IPSIAVPLSLVGTFAVMYVCGFSLNNLTLMALTIATGFVVDDAIVMLENIARHLEEGETP
LNAALKGARQIGFTLISLTFSLIAVLIPLLFMQDVVGRLFREFAITLAVAILISLVVSLT
LTPMMCAKLLKPHSVAEAKPDWVERLIGGYSRWLTWVLRHQTLTLLVAVATLGLTVVLYL
AVPKGFFPVQDTGVIQGISEAPQSISFRAMSERQQALARVILADPSVQSLSSYIGVDGDN
VTLNSGRLLINLKPHGERDLTASQIIDRLRPELAKVPGIELYLQPVQDLSIEDRVSRTQF
QFSLETPDSELLQEWTPRLVEALRERPELTDVASDLQSNGLQIYLDIDRDAAARLGIQVA
DITDALYDAFGQRQISTIFTQASQYRVVLEAEAGNRLGPQALEQLFVQSEGGTPVRLSSL
ATFEQRNAPLLINHIGQFPAVTLSFNLASGVSLGKAVEVIEAVEQQIGLPAGIQTRFQGA
AEAFRASLSSTLLLIFAAVVTMYIVLGVLYESYIHPITILSTLPSAAVGALLALLLTGND
LGLIAIIGIILLIGIVKKNAIMMIDFALEAERHQGMSPQDAIYRAALLRFRPILMTTLAA
LFGAIPLMLASGSGAELRQPLGLVLVGGLLLSQLLTLFTTPVIYLFFDRLGQRFGRRREP
LLEAEA