Protein Info for Psest_2134 in Pseudomonas stutzeri RCH2

Annotation: amino acid carrier protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 17 to 37 (21 residues), see Phobius details amino acids 71 to 92 (22 residues), see Phobius details amino acids 99 to 120 (22 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 183 to 203 (21 residues), see Phobius details amino acids 212 to 237 (26 residues), see Phobius details amino acids 245 to 268 (24 residues), see Phobius details amino acids 307 to 330 (24 residues), see Phobius details amino acids 350 to 373 (24 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 419 to 439 (21 residues), see Phobius details TIGR00835: amino acid carrier protein" amino acids 16 to 443 (428 residues), 488.5 bits, see alignment E=8.3e-151 PF01235: Na_Ala_symp" amino acids 58 to 446 (389 residues), 461.7 bits, see alignment E=1.3e-142

Best Hits

Swiss-Prot: 52% identical to AGCS_METMP: Sodium/alanine symporter AgcS (agcS) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K03310, alanine or glycine:cation symporter, AGCS family (inferred from 96% identity to psa:PST_2182)

Predicted SEED Role

"Sodium/glycine symporter GlyP" in subsystem Glycine cleavage system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIV4 at UniProt or InterPro

Protein Sequence (447 amino acids)

>Psest_2134 amino acid carrier protein (Pseudomonas stutzeri RCH2)
MEFLNNLVNSVNGLVWGPPMLVLILGTGLFLMIFLKFMPLTRIPTGFALMWRGRTKGDEA
TGEISPFQALMTSLAATVGTGNIAGVATAIFLGGPGALFWMWCTALVGMATKYCEVVLAV
HYREKDDRGEHVGGPMYAIKNGLGKKWIWLGTAFAIFGGLAGFGIGNMVQVNSMAHALET
TFAVPLWATGLVTMVIVGLVILGGIRRIGVVAASLVPFMCLAYLIAAAVVLVVNVSAIPA
AFDLIFTHAFTPIAATGGFAGAAVMAAIRFGVARGIFSNEAGLGTAGIAQAAGTTTSSVR
SGMIGMLGTFIDTIIVCSMTGLAIICTGVWTSGESGAALSAAAFESAMPGIGGIILTIAL
VVFAFTTILGWSYFGEKCWEFLVGTRAIWPFRVIWVLAVPFGAIAQLDFAWLLADTLNGL
MAIPNLISLLLLSPVVVKLTKEYFARS