Protein Info for Psest_2124 in Pseudomonas stutzeri RCH2

Updated annotation (from data): D-gluconate TRAP transporter, large permease component
Rationale: specific phenotype on D-gluconate and no other transporter is apparent in the fitness data.
Original annotation: TRAP transporter, DctM subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 transmembrane" amino acids 9 to 35 (27 residues), see Phobius details amino acids 54 to 73 (20 residues), see Phobius details amino acids 94 to 123 (30 residues), see Phobius details amino acids 133 to 151 (19 residues), see Phobius details amino acids 170 to 192 (23 residues), see Phobius details amino acids 212 to 234 (23 residues), see Phobius details amino acids 240 to 256 (17 residues), see Phobius details amino acids 277 to 296 (20 residues), see Phobius details amino acids 315 to 343 (29 residues), see Phobius details amino acids 355 to 379 (25 residues), see Phobius details amino acids 399 to 424 (26 residues), see Phobius details PF06808: DctM" amino acids 9 to 415 (407 residues), 397.6 bits, see alignment E=3.1e-123 TIGR00786: TRAP transporter, DctM subunit" amino acids 17 to 419 (403 residues), 438.6 bits, see alignment E=9.9e-136

Best Hits

Swiss-Prot: 55% identical to YIAN_ECOLI: 2,3-diketo-L-gulonate TRAP transporter large permease protein YiaN (yiaN) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to psa:PST_2200)

MetaCyc: 55% identical to 2,3-diketo-L-gulonate:Na+ symporter - membrane subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-235

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, large permease component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIU4 at UniProt or InterPro

Protein Sequence (425 amino acids)

>Psest_2124 D-gluconate TRAP transporter, large permease component (Pseudomonas stutzeri RCH2)
MTVVVFLSSLLGFMTLGMPIAFALLLTGSVLMWYLDFWDVQLLAQNLQAGADSFPLLAVP
FFILAGELMNAGGISRRIIAMAQAYFGHKRGGLGYVAIAASVLLASMSGSALADTAALAT
LLLPMMRERGYPLSSSSGLVAAGGIIAPIIPPSMPFVIYGVVTGTSISQLFLAGMVPGLI
MGMGLIVAWTLIARRIDEPKQEKASAAERRRVLVDGAAALMLPVIIVGGLRGGLFTPTEA
AVVAAVYALAVSTLLYRELNWAGLVEVLTRASRTTASVMFLCAAATVSAYMITLAQLPDE
IAAMLGPLAQDPKLLMVAIMLLMIAVGMVLDLTPTILILGPVLAPIAIKAGIDPVYFGVM
FVLIGSIGLITPPVGTVLNVVGGIGRLRMETLVRGVMPFFLIYLVIVGLLIAVPSIITVP
LAWLR