Protein Info for PGA1_c02110 in Phaeobacter inhibens DSM 17395

Annotation: tRNA 2-thiocytidine biosynthesis protein TtcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 PF01171: ATP_bind_3" amino acids 53 to 216 (164 residues), 52.3 bits, see alignment E=3.1e-18

Best Hits

Swiss-Prot: 92% identical to TTCA_RUEST: tRNA-cytidine(32) 2-sulfurtransferase (ttcA) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K14058, tRNA 2-thiocytidine biosynthesis protein TtcA (inferred from 92% identity to sit:TM1040_0309)

Predicted SEED Role

"tRNA(Cytosine32)-2-thiocytidine synthetase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DWY9 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PGA1_c02110 tRNA 2-thiocytidine biosynthesis protein TtcA (Phaeobacter inhibens DSM 17395)
MLDDDLDDIHPLFAGAPSTTEFKKLRKRIVRYAREAIDQYGMVERREDGSTPKWLVCLSG
GKDSYTLLAVLYELKWRGLLPVDLLACNLDQGQPGFPATVLPEFLEKMGVPHRIEYQDTY
SIVMDKVPQGRTFCALCSRLRRGNLYRIAREEGCSAVVLGHHRDDILETFFMNLFHGGRL
ATMPPKLVNEEGDLFVFRPLAHVAEVDCEKFAKALNYPIIPCDLCGSQDGLQRQQVKQIL
DQWESNSPGRRQVMFRALMNARPSHLLDPKLFDFSALMLKNTDLGADSDEIPDLR