Protein Info for Psest_2112 in Pseudomonas stutzeri RCH2

Annotation: Methyl-accepting chemotaxis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 544 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 192 to 216 (25 residues), see Phobius details PF08269: dCache_2" amino acids 32 to 186 (155 residues), 149.2 bits, see alignment E=3.7e-47 PF17200: sCache_2" amino acids 42 to 189 (148 residues), 140.6 bits, see alignment E=1.1e-44 PF17201: Cache_3-Cache_2" amino acids 96 to 188 (93 residues), 35.7 bits, see alignment E=1.6e-12 PF00672: HAMP" amino acids 211 to 263 (53 residues), 52.3 bits, see alignment 1.4e-17 PF00015: MCPsignal" amino acids 331 to 509 (179 residues), 144.5 bits, see alignment E=7.8e-46

Best Hits

Swiss-Prot: 62% identical to MCPP_PSEPK: Methyl-accepting chemotaxis protein McpP (mcpP) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03406, methyl-accepting chemotaxis protein (inferred from 67% identity to pmk:MDS_2547)

Predicted SEED Role

"Methyl-accepting chemotaxis protein I (serine chemoreceptor protein)" in subsystem Bacterial Chemotaxis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKX3 at UniProt or InterPro

Protein Sequence (544 amino acids)

>Psest_2112 Methyl-accepting chemotaxis protein (Pseudomonas stutzeri RCH2)
MNLLRNVPISKRLWLIPVVAVVMLFILGLLMIQQVRSDLYRGKQESTRHVVEAASGVFEH
YRKLELSGAMTTAEAQKAAIEQIRLLRYDGQDYYWINDLGPTMIMHPMQPKLDGQDLSKI
KDPEGKELFNEMVRIAQRDGAGLVDYMWAKPGEENPVPKISYVQLFKPWGWIIGSGIYVD
DVEQEFWNYLTRFSLIGLLISAIMAVLVAILIRSIVRPLRHTMAAMANIASGEADLTRTL
DVSGNDELTTLGRDFNRFTQKLRTVVGQLFETAGALDQSSRSLSQLSGQAHEQSQQQMLQ
MEQVATAINEVTYAVQEVAKNAEHASSEVGSAENQAGQGQRNIETSLQQIDELSATIGQA
VDVIQSLADETTKIGSVLEVIGSIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRLLA
QRTQQSTAEIHGMIERLQKNSGAAVNVIMESNRASQLTVEQASQAGESLGEIAQALRNLS
GLNASIASATLQQSHVVEDINQNVTQAAGLAQESTLAAEQSSEAGKQLGELAGQLNRLLS
QFRV