Protein Info for Psest_2086 in Pseudomonas stutzeri RCH2

Annotation: putative quinone oxidoreductase, YhdH/YhfP family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 TIGR02823: putative quinone oxidoreductase, YhdH/YhfP family" amino acids 5 to 329 (325 residues), 440.6 bits, see alignment E=1.3e-136 PF08240: ADH_N" amino acids 31 to 117 (87 residues), 51.9 bits, see alignment E=7.9e-18 PF00107: ADH_zinc_N" amino acids 162 to 274 (113 residues), 46.1 bits, see alignment E=4.8e-16

Best Hits

Swiss-Prot: 45% identical to ACUI_RUEPO: Acrylyl-CoA reductase AcuI (acuI) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K00001, alcohol dehydrogenase [EC: 1.1.1.1] (inferred from 93% identity to psa:PST_2239)

MetaCyc: 45% identical to acryloyl-CoA reductase monomer (Ruegeria pomeroyi DSS-3)
RXN-9087 [EC: 1.3.1.84]

Predicted SEED Role

"Alcohol dehydrogenase (EC 1.1.1.1)" in subsystem Fermentations: Mixed acid or Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 1.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1

Use Curated BLAST to search for 1.1.1.1 or 1.3.1.84

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMK7 at UniProt or InterPro

Protein Sequence (330 amino acids)

>Psest_2086 putative quinone oxidoreductase, YhdH/YhfP family (Pseudomonas stutzeri RCH2)
MSSFKALQVSEVADGRFESKVVERGIDELPAGEVLIRVRYSSLNFKDALSASGNRGVTRS
YPHTPGIDAAGVVASSSVGEFAEGDEVIVTGYDLGMNTAGGFGQYIRVPAAWVIKRPQGL
SLRDAMILGTAGLTAALCVDKLEQAGLEPGDAPVLVTGATGGVGSIAVALLASLGYKVAA
VTGKADQAAFLTRLGASQIVERSALQAGVEKALLKEQWGGAVDTVGGDILFNVVKSLQRG
ASVACCGLTAGTHFQASVLPFILRGVNLLGVDSVEIPLVVKASMWDKLSLQWKLVNLEDL
THEIALEQLPEAIERILAGGQVGRILVRVD