Protein Info for PGA1_c20740 in Phaeobacter inhibens DSM 17395
Annotation: uroporphyrinogen-III C-methyltransferase CobA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02303, uroporphyrin-III C-methyltransferase [EC: 2.1.1.107] (inferred from 79% identity to sit:TM1040_1756)MetaCyc: 44% identical to CobA (Pseudomonas denitrificans (nom. rej.))
Uroporphyrinogen-III C-methyltransferase. [EC: 2.1.1.107]; 2.1.1.107 [EC: 2.1.1.107]
Predicted SEED Role
"Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase or Experimental tye or Heme and Siroheme Biosynthesis (EC 2.1.1.107)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (13/13 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (11/15 steps found)
- siroheme biosynthesis (3/4 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (24/36 steps found)
- factor 430 biosynthesis (3/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.107
Use Curated BLAST to search for 2.1.1.107
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7E227 at UniProt or InterPro
Protein Sequence (277 amino acids)
>PGA1_c20740 uroporphyrinogen-III C-methyltransferase CobA (Phaeobacter inhibens DSM 17395) MTGPDTIPPRARLPMQGVACVSVSSSLVAGEVAFAGSGPGDPDLLTLKVARALLQADVIL FDRLVSAEIMALARPQAQLEDVGKEGFGPQVSQEEICVRMVAHARAGKKVLRLKSGDPTV YGRLDEELTACEAAGVAYQILPGITAASAAVASIGQSLTQRGRNASVRFLTGHDMKGFAD HDWAALARPGEVAAIYMGKKSARFVQGRLIMHGADRATPMTIVENASRANQRVIETTLER LPTDLAAAELTGPALTFLGLAPRASAQALTNLKLELA