Protein Info for Psest_2064 in Pseudomonas stutzeri RCH2

Annotation: ABC-type uncharacterized transport system, duplicated ATPase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 553 PF00005: ABC_tran" amino acids 31 to 190 (160 residues), 108.4 bits, see alignment E=1.1e-34 amino acids 307 to 457 (151 residues), 111.9 bits, see alignment E=9.7e-36 PF08352: oligo_HPY" amino acids 241 to 268 (28 residues), 21.3 bits, see alignment (E = 7.4e-08) amino acids 508 to 533 (26 residues), 24.7 bits, see alignment (E = 6.1e-09)

Best Hits

Swiss-Prot: 60% identical to YEJF_ECOLI: Uncharacterized ABC transporter ATP-binding protein YejF (yejF) from Escherichia coli (strain K12)

KEGG orthology group: K13896, microcin C transport system ATP-binding protein (inferred from 94% identity to psa:PST_2261)

MetaCyc: 60% identical to putative oligopeptide ABC transporter ATP binding subunit YejF (Escherichia coli K-12 substr. MG1655)
3.6.3.23-RXN [EC: 7.4.2.6]

Predicted SEED Role

"Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) (TC 3.A.1.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIP3 at UniProt or InterPro

Protein Sequence (553 amino acids)

>Psest_2064 ABC-type uncharacterized transport system, duplicated ATPase component (Pseudomonas stutzeri RCH2)
MAEQPIAENLVEVRDLAVEFVTGEQVQRVVEGVTFDIRKGETLALVGESGSGKSVTAHSI
LRLLPYPLARHPQGQILFHGQDLLKADEKAMRKIRGNRIAMVFQEPMTSLNPLHTVGKQI
NEVLEIHKGLRGKAATARTLELLELVGIPEPRKRIRAYPHELSGGQRQRVVIAMALANEP
ELLIADEPTTALDVTVQLKILELLKELQARLGMALLLISHDLNLVRRIAHRVCVMQRGRV
VEQALCEDLFRAPQHPYTQELLAAEPSGGPVAVEEAPPLLEVDDLRVWFPIKKGLLRRTV
DHIKAVDGVNFSLPKGQTLGIVGESGSGKSTLGLAILRLLGSRGEIRFQGQHLQSMSQRQ
VRPLRRQMQVVFQDPFGSLSPRMSVGQIVGEGLHIHRMGNAKEQEQAIIEALVEVGLDPE
TRHRYPHEFSGGQRQRIAIARALVLKPALILLDEPTSALDRTVQRQVVELLRSLQAKYNL
TYLFISHDLAVVRALSHQMMVVKQGQVVEQGAAADIFAAPQHPYTQQLLESAFMAPGTAE
QPEEGQAHGFSHR