Protein Info for Psest_2047 in Pseudomonas stutzeri RCH2

Annotation: Signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 133 to 155 (23 residues), see Phobius details PF00672: HAMP" amino acids 168 to 205 (38 residues), 29.8 bits, see alignment 9.3e-11 PF07730: HisKA_3" amino acids 224 to 288 (65 residues), 46.7 bits, see alignment E=5.6e-16

Best Hits

KEGG orthology group: K07675, two-component system, NarL family, sensor histidine kinase UhpB [EC: 2.7.13.3] (inferred from 82% identity to psa:PST_2278)

Predicted SEED Role

"Nitrate/nitrite sensor protein (EC 2.7.3.-)" in subsystem Nitrate and nitrite ammonification (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKR9 at UniProt or InterPro

Protein Sequence (421 amino acids)

>Psest_2047 Signal transduction histidine kinase (Pseudomonas stutzeri RCH2)
MSALWRINLLVTLFFTLVTLVCLAVLLRQASHDVQRELNAAAAVVGYLGEMAERDPDSLK
PGLTDSLRHVRVRWLEAELVGTAAADPVRSDWLGAWLYPAELSAPTVLQLPNGRRLHISV
DPSDEVEEVRDSLVQLVVLFGLALLLCLLAIRWALRPGMRVLAELFGALVRIADGRLDTR
LPPHRFAEARELAEHFNHMAAALEDARLENERLTQALLALQERERTRLAQALHDDLGQYL
AGIRARVCLLRVQADQPSAIRETAAHLEQHSLDLQNGFRALVRDLYPVMLDHLSLEDAIG
QLAEQWQSTQGIACEVRLRGPIPDLSMDAKVHLYRMVQEALTNVARHARATRVCLDLRGD
PLGLRLLIHDDGHGQPAERPGVGLRSMVEHARCLGARLRVRHRAGKGWALYLKLPLQGAI
S