Protein Info for Psest_2037 in Pseudomonas stutzeri RCH2

Annotation: uroporphyrin-III C-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 207 (204 residues), 267.4 bits, see alignment E=7.9e-84 PF13241: NAD_binding_7" amino acids 6 to 115 (110 residues), 101.7 bits, see alignment E=6.5e-33 PF14824: Sirohm_synth_M" amino acids 119 to 144 (26 residues), 32 bits, see alignment (E = 1.4e-11) PF10414: CysG_dimeriser" amino acids 150 to 207 (58 residues), 71.6 bits, see alignment 7.4e-24 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 216 to 451 (236 residues), 317.1 bits, see alignment E=8.5e-99 PF00590: TP_methylase" amino acids 218 to 428 (211 residues), 193 bits, see alignment E=1.3e-60

Best Hits

Swiss-Prot: 94% identical to CYSG_PSEU5: Siroheme synthase (cysG) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 94% identity to psa:PST_2288)

MetaCyc: 56% identical to siroheme synthase (Escherichia coli K-12 substr. MG1655)
Sirohydrochlorin ferrochelatase. [EC: 4.99.1.4]; Uroporphyrinogen-III C-methyltransferase. [EC: 4.99.1.4, 2.1.1.107]; 2.1.1.107 [EC: 4.99.1.4, 2.1.1.107]; 2.1.1.107 [EC: 4.99.1.4, 2.1.1.107]; Precorrin-2 dehydrogenase. [EC: 4.99.1.4, 2.1.1.107, 1.3.1.76]

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.107

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKR2 at UniProt or InterPro

Protein Sequence (464 amino acids)

>Psest_2037 uroporphyrin-III C-methyltransferase (Pseudomonas stutzeri RCH2)
MEYLPLFHNLKGRTVLIVGGGEIALRKARLLSEAGARLRVVAPSIEPQLVELVEAGGGEC
LNRGYDPQDLQGCVLAIAATDDEPLNALVSQHANALGMPVNVVDSPELCSVIFPAIVDRS
PLVIAVSSGGDAPVLARLIRARIETWIPAAYGQLAGLAKQFRAQVKAKFANVQQRRVFWE
ETFQGPIAEQALAGRSVEAERLLAEKLAGAAPRALGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPAIIDLCRRDADRIYVGKQRSEHAVPQEQINQKLVTLAKEGKRVLRLK
GGDPFIFGRGGEEIEELAAHGVPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHL
KDGSCDLPWAELAAPAQTLVFYMGLVGLPVICQQLIAHGRAAGTPAALVQQGTTSNQRVF
TGTLETLAELIAQEQVQAPTLLIVGEVVQLREKLAWFEGAQASA