Protein Info for Psest_2027 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent Clp protease ATP-binding subunit clpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 756 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 739 (737 residues), 1098.5 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 53.6 bits, see alignment 1.5e-17 PF13191: AAA_16" amino acids 191 to 239 (49 residues), 35.1 bits, see alignment 1.4e-11 PF00004: AAA" amino acids 215 to 346 (132 residues), 45.6 bits, see alignment E=7.4e-15 amino acids 496 to 611 (116 residues), 49.8 bits, see alignment E=3.7e-16 PF17871: AAA_lid_9" amino acids 354 to 456 (103 residues), 96.5 bits, see alignment E=6.1e-31 PF07724: AAA_2" amino acids 490 to 651 (162 residues), 206.2 bits, see alignment E=2.6e-64 PF00158: Sigma54_activat" amino acids 494 to 616 (123 residues), 24.6 bits, see alignment E=1.4e-08 PF07728: AAA_5" amino acids 495 to 613 (119 residues), 52.7 bits, see alignment E=3.6e-17 PF10431: ClpB_D2-small" amino acids 657 to 737 (81 residues), 95.8 bits, see alignment E=9.9e-31

Best Hits

Swiss-Prot: 66% identical to CLPA_ECO57: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O157:H7

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 98% identity to psa:PST_2298)

MetaCyc: 66% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKQ3 at UniProt or InterPro

Protein Sequence (756 amino acids)

>Psest_2027 ATP-dependent Clp protease ATP-binding subunit clpA (Pseudomonas stutzeri RCH2)
MLNRELEVTLNLAFKEARTKRHEFMTVEHLLLALLDNEAAATVLRACGASLDKLRHDLQE
FIDSTTPLIPQHDEERETQPTLGFQRVLQRAVFHVQSSGKREVTGANVLVAIFSEQESQA
VFLLKQQNVARIDVVNYIAHGISKVPGNAGSPESDAEMQDEDGGESGASSNPLDAYASNL
NELARQGRIDPLVGRENEVERVAQILARRRKNNPLLVGEAGVGKTAIAEGLAKRIVDNQV
PDLLADSVVYSLDLGALLAGTKYRGDFEKRFKALLGELRKRPHAILFIDEIHTIIGAGAA
SGGVMDASNLLKPLLSSGEIRCIGSTTFQEFRGIFEKDRALARRFQKVDVSEPSVEDTIG
ILRGLKGRFEQHHHIEYSDEALRAAAELASRYINDRHMPDKAIDVIDEAGAYQRLQPEDQ
RVKCIDVEQVEDIVAKIARIPPKHVSSSDKELLRNLERDLKLTVFGQDDAIDSLATAIKL
SRAGLKAPDKPVGSFLFAGPTGVGKTEAARQLARALGVELIRFDMSEYMERHTVSRLIGA
PPGYVGFDQGGLLTEAITKQPHCVLLLDEIEKAHPEVFNLLLQVMDHGTLTDNNGRKADF
RNVIIIMTTNAGAETAARASIGFTLQDHASDAMEVIKKSFTPEFRNRLDTIIQFGRLSHE
VIKSIVDKFLIELQAQLEDKHVTLEVSDAARGWLAEHGYDAQMGARPMARLIQDKIKRPL
AEEILFGELAEHGGVVHIDIRDGEPFFEFETSAELA