Protein Info for Psest_2022 in Pseudomonas stutzeri RCH2

Annotation: ADP-ribose pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00293: NUDIX
  • amino acids 6 to 125 (120 residues), 67.5 bits, E=6.4e-23

Best Hits

Swiss-Prot: 42% identical to NUDJ_ECOLC: Phosphatase NudJ (nudJ) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: None (inferred from 88% identity to psa:PST_2303)

MetaCyc: 42% identical to phosphatase NudJ (Escherichia coli K-12 substr. MG1655)
RXN0-7309 [EC: 3.6.1.76]; Guanosine-diphosphatase. [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]; 3.6.1.- [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]; 3.6.1.- [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]

Predicted SEED Role

"Nudix-like NDP and NTP phosphohydrolase YmfB" in subsystem Nudix proteins (nucleoside triphosphate hydrolases)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• prenylated FMNH₂ biosynthesis (2/3 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.42 or 3.6.1.5 or 3.6.1.76

Sequence Analysis Tools

See L0GKQ0 at UniProt or InterPro

Protein Sequence (146 amino acids)

>Psest_2022 ADP-ribose pyrophosphatase (Pseudomonas stutzeri RCH2)
MDWQPHITVATVIENQGRFLLVEELKAGRLVLNQPAGHLEANENLREAAVRETLEETGWD
VELTGVIGIYLYTAPSNGVTYQRVCFAAKALQHDPQRTLDEGIVAARWLSREELAEQPDR
WRSELVPRCIDDYLSGPLHSLEVIRN