Protein Info for PGA1_c20010 in Phaeobacter inhibens DSM 17395

Annotation: glutamate-ammonia-ligase adenylyltransferase GlnE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 938 PF03710: GlnE" amino acids 43 to 275 (233 residues), 167.9 bits, see alignment E=2.9e-53 amino acids 527 to 761 (235 residues), 175.8 bits, see alignment E=1.1e-55 PF08335: GlnD_UR_UTase" amino acids 297 to 437 (141 residues), 83.7 bits, see alignment E=1.3e-27 amino acids 812 to 920 (109 residues), 25.2 bits, see alignment E=1.5e-09

Best Hits

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 73% identity to sil:SPO2523)

Predicted SEED Role

"Glutamate-ammonia ligase adenylyltransferase (EC 2.7.7.42)" (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DRJ2 at UniProt or InterPro

Protein Sequence (938 amino acids)

>PGA1_c20010 glutamate-ammonia-ligase adenylyltransferase GlnE (Phaeobacter inhibens DSM 17395)
MGQSIEIQRLPRAFDADLGQDALALVPALHGDQARLVAGAAGSSPYLKELISREADWLAP
ALADPQTALAGIFADVRALAPDQLKPGLRRAKRQLALLSGLCDLAGAWDLMQVTTALTQF
AELCADVAIKAEIAALIRRKKLPGLTEADVDTAGGLTLLAMGKMGAYELNYSSDIDMICL
FDETRFDPDDFYEARQGMVRATRNMCTVLSERTADGYVFRTDLRLRPDPSVTPVAMAMEA
AERYYESLGRTWERAAYIKARPCAGDLKAGADFLQTLRPFVWRRHLDFAAIQDAHDMRLR
IRENKGTGGPLTVPGHDMKLGRGGIREIEFFTQTRQLIAGGRDESLRPRGTLDGLRALAA
KDWVPQDVCDQLGAHYVAHREVEHRIQMVHDAQTHRMPQSEDGIARIACLMDRDPAALLA
QTKARLQEVHELTEGFFAPDAPPEVAPQTLPDALDSDILARWPTYPALRSARGAQIFERL
KPELLARLSRTAQPTEALVALDGFLAGLPAGVQLFSLFEANPQLIDLLIDIVGTSGTLAS
YLSRNSSVFDAVIGGSFFDDWPGREALGQLLAVVLAQEEDYETQLDATRRWCKEWHFRIG
VHHLRGLIDAATAGAQYAELAEVVIAGLAPVVVQQFAQKHGPPPGRGAAVLGMGSLGAGR
INALSDLDVIVIYDPGTAEASDGPRPLATRPYYARLTQALITALTARMSQGQLYEVDMRL
RPSGTQGPVATSLESFCQYQRNDAWVWEHLALTRARVVAIAPLPAAAGLAQDIAAFRRTF
LAAPRSRDAILPDVAKMRARLAAAKAPSGPWDAKTGAGRMMDIELIAQAGVLLSEATGQD
VAAGLEGAVACGWLDAADAAELQRGYDLFWSVQTAARLVSGKAIDAQTLGEGGALFLCRS
TGYADLAALEADLTARYARCGDIIAAALEREGASGHDD