Protein Info for Psest_2001 in Pseudomonas stutzeri RCH2

Annotation: 2-phosphoglycolate phosphatase, prokaryotic

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 PF00702: Hydrolase" amino acids 4 to 180 (177 residues), 114 bits, see alignment E=2.4e-36 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 6 to 214 (209 residues), 175.5 bits, see alignment E=1.6e-55 PF12710: HAD" amino acids 6 to 174 (169 residues), 40.2 bits, see alignment E=1.1e-13 PF13419: HAD_2" amino acids 6 to 186 (181 residues), 117.8 bits, see alignment E=1.3e-37 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 77 to 183 (107 residues), 39.5 bits, see alignment E=9.5e-14 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 80 to 180 (101 residues), 41.1 bits, see alignment E=3.6e-14 PF13242: Hydrolase_like" amino acids 141 to 208 (68 residues), 45.5 bits, see alignment E=1.1e-15

Best Hits

Swiss-Prot: 77% identical to MUPP_PSEPK: N-acetylmuramic acid 6-phosphate phosphatase (mupP) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 92% identity to psa:PST_2324)

MetaCyc: 77% identical to N-acetyl-beta-muramate 6-phosphate phosphatase (Pseudomonas putida KT2440)
RXN-18659 [EC: 3.1.3.105]

Predicted SEED Role

"Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.105 or 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GME6 at UniProt or InterPro

Protein Sequence (223 amino acids)

>Psest_2001 2-phosphoglycolate phosphatase, prokaryotic (Pseudomonas stutzeri RCH2)
MRLRAVLFDMDGTLLDTAPDFIAVAQAMRVARGLAPVPAQQVRDVVSGGARAMVLSAFDV
DPLSDEFEELRLEFLERYQEHCAVHSHLYDGMAELLDEIERSNLLWGVVTNKPLRFAEPI
MHQLGLASRSAVLVCPDHVARSKPDPEPMLLACKQLDLDPSAVLFVGDDLRDIESGRAAG
SRTAAVRYGYIHPEDNPSNWGADVVVDHPLELRKVLDRAICSC