Protein Info for Psest_1999 in Pseudomonas stutzeri RCH2

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 PF01479: S4" amino acids 16 to 60 (45 residues), 46 bits, see alignment 3.4e-16 PF00849: PseudoU_synth_2" amino acids 79 to 210 (132 residues), 68.1 bits, see alignment E=1e-22 TIGR00093: pseudouridine synthase" amino acids 83 to 245 (163 residues), 177.1 bits, see alignment E=1.1e-56

Best Hits

Swiss-Prot: 74% identical to RLUB_PSEAE: Ribosomal large subunit pseudouridine synthase B (rluB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 74% identity to pfv:Psefu_2006)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)" in subsystem Two cell division clusters relating to chromosome partitioning (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GII6 at UniProt or InterPro

Protein Sequence (377 amino acids)

>Psest_1999 pseudouridine synthase (Pseudomonas stutzeri RCH2)
MTEHEETSTHVTHGEKLQKVLARLGMASRRDVEKWIVEGRVKVNGAVASLGQRVDSHDAI
AVDGRLLKREEANEVVRRVLIYNKPDGEICTRNDPEGRPTVFDRLPRPKEGRWINIGRLD
INTTGLLLFTTDGELANRLMHPSYEMDREYAVRVRGEVDDEMIERLKTGVMLEDGPARFT
DIQEAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFMTSDLPMGRW
REMSQSEVDILSQEVGLKPVALPSMKAKAKEKIDRMQRKVAKPLGRGERRPRVLRSSTEA
DVAGSDKPRVQRDDQAGRKPRVNEGRSDKTRGTPVAERPGDARKGGKRNDGAGRPSGPGK
RPAGDGQRPGFGRSKRT