Protein Info for Psest_1995 in Pseudomonas stutzeri RCH2

Annotation: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 8 to 289 (282 residues), 424.6 bits, see alignment E=1.8e-131 TIGR00536: methyltransferase, HemK family" amino acids 10 to 283 (274 residues), 272.2 bits, see alignment E=4.5e-85 PF17827: PrmC_N" amino acids 23 to 90 (68 residues), 24.4 bits, see alignment E=1.8e-08 PF03602: Cons_hypoth95" amino acids 127 to 209 (83 residues), 27.7 bits, see alignment E=1.1e-09 PF10294: Methyltransf_16" amino acids 128 to 184 (57 residues), 22.8 bits, see alignment E=3.4e-08 PF05175: MTS" amino acids 129 to 209 (81 residues), 61.8 bits, see alignment E=3.3e-20 PF06325: PrmA" amino acids 129 to 202 (74 residues), 26.4 bits, see alignment E=2.3e-09 PF13847: Methyltransf_31" amino acids 129 to 213 (85 residues), 39.3 bits, see alignment E=2.8e-13 PF13649: Methyltransf_25" amino acids 131 to 211 (81 residues), 34.8 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 80% identical to PRMB_PSEAE: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 95% identity to psa:PST_2330)

MetaCyc: 54% identical to ribosomal protein L3 N5-glutamine methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-1241 [EC: 2.1.1.298]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.298 or 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMI4 at UniProt or InterPro

Protein Sequence (307 amino acids)

>Psest_1995 protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (Pseudomonas stutzeri RCH2)
MTASLSRLRTLRDYIRWAVSRFQAEQLFFGHGTDNAWDDARQLVLGALHLPWEMSDSYLD
CRLEQDECEHLQNLLHRRIDERVPTAYLLGQAWFCGLPFIVDERVLIPRSPIGELIEHRF
EPWLPGTPKRILDLCTGSGCIGIACAYEFLEAEVVLADLSFDAIEVANRNVEQHGLEDRV
YTVQSDGFDGLPNQRFDLIVSNPPYVDAEDFGDMPDEFHHEPALGLACGEDGLDLVRRML
AEAADHLTDDGTLIVEVGNSQIHVQALYPEVDFTWLEFSRGGHGVFLLAAQQCRKHQALF
RSRLEKH