Protein Info for Psest_1983 in Pseudomonas stutzeri RCH2

Annotation: phosphoserine aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 93% identical to SERC_PSEST: Phosphoserine aminotransferase (serC) from Pseudomonas stutzeri

KEGG orthology group: K00831, phosphoserine aminotransferase [EC: 2.6.1.52] (inferred from 95% identity to psa:PST_2342)

MetaCyc: 52% identical to phosphoserine aminotransferase (Arabidopsis thaliana col)
Phosphoserine transaminase. [EC: 2.6.1.52]

Predicted SEED Role

"Phosphoserine aminotransferase (EC 2.6.1.52)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 2.6.1.52)

MetaCyc Pathways

superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
L-serine biosynthesis I (3/3 steps found)
L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (8/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.52

Use Curated BLAST to search for 2.6.1.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL71 at UniProt or InterPro

Protein Sequence (361 amino acids)

>Psest_1983 phosphoserine aminotransferase (Pseudomonas stutzeri RCH2)
MSKRAFNFCAGPAALPEAVLQRAQAELLDWHGKGLSVMEMSHRSDEYTAIAEKAEQDLRD
LLAIPSNYKVLFLQGGASQQFAEIPLNLLPEDGVADYIDTGIWSRKAIEEARRFGNVNVA
ASAKPLDYFAIPGQNDWQLSERAAYLHYASNETIGGLQFDWVPEVGDTPLVVDMSSDILS
RTIDVSKFGLIYAGAQKNIGPSGLVVVIVREDLLGRARSACPTMLDYKIAADNGSMYNTP
ATFSWYLSGLVFEWLKEQGGVEAMEQRNRAKKDMLYGAIDASDFYTNPIAVNARSWMNVP
FRLADERLDKSFLAGADARGLLNLKGHRSVGGMRASIYNAVGLDAVEALVAYMAEFEKEH
G