Protein Info for Psest_1978 in Pseudomonas stutzeri RCH2

Annotation: DNA-directed DNA polymerase III (polc)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1026 TIGR00594: DNA polymerase III, alpha subunit" amino acids 6 to 971 (966 residues), 562.2 bits, see alignment E=1.3e-172 PF02811: PHP" amino acids 7 to 125 (119 residues), 65.4 bits, see alignment E=2.1e-21 PF07733: DNA_pol3_alpha" amino acids 270 to 525 (256 residues), 300.9 bits, see alignment E=2.2e-93 PF17657: DNA_pol3_finger" amino acids 528 to 693 (166 residues), 196.9 bits, see alignment E=4e-62 PF14579: HHH_6" amino acids 767 to 856 (90 residues), 75.5 bits, see alignment E=7.9e-25 PF01336: tRNA_anti-codon" amino acids 936 to 1008 (73 residues), 34.4 bits, see alignment 4.2e-12

Best Hits

Swiss-Prot: 79% identical to DNAE2_PSEMY: Error-prone DNA polymerase (dnaE2) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 96% identity to psa:PST_2347)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL66 at UniProt or InterPro

Protein Sequence (1026 amino acids)

>Psest_1978 DNA-directed DNA polymerase III (polc) (Pseudomonas stutzeri RCH2)
MNTEYAELHCLSNFSFQRGASSARELFERAKRQGYDALAITDECSLAGIVRAWQASKDTG
LPLIIGSELQIEGGPKLVLLVENLTGYQALCQLITRARRRAEKGRYQALREDLRDWPLEG
LLAIWLPDHDGADAEGRWLLDRFRERLWLGIELHRGPDDALQLRQSLTQASRLGISAVAC
GDVHMHARGRRALQDCMTAIRHHLPVAEAGRHLFPNGERHLRSREELAQLYPAELLAETL
RISQRCHFQLDQLKYHYPHELVPPGHDTTSWLRKLVEDGARWRWPDGIPEKARAQLEHEL
ELIADLHYESYFLTVHDIVRFAREQGILCQGRGSAANSSVCFVLGITELDPVRSKLLFER
FLSRERNEPPDIDVDFEHDRREEVIQYVFQRYGRNRAALTAVASSYRATGALRDVAKALG
LPPDQINALAGCCGRWSEHIPDDSQLREAGFDPDNPVLRRVLVLTDALIGFPRHLSQHPG
GFVISEQPLDTLVPIENASMADRTVIQWDKDDLDAVGLLKVDVLALGMLSAIRRCFDLIE
HYRGTRWTIATLPAEDKPTYEMISRADTIGVFQIESRAQMAMLPRLRPKTFYDLVIQVAI
VRPGPIQGDMVHPYLRRRNKEEDVDYPSEELKPVFERTLGVPLFQEQVMELAIVAAEYTP
DEADKLRRAMAAWKRHGSLEPHRVRLTERMLAKGYQPDFIARIFEQIKGFGSYGFPESHA
ASFALLTYASCWLKRHEPAAFACALINSWPMGFYNPDQILQDARRHGLEIRPVDVRHSGW
DCSLEPCSQEQPAIRLGLRMVRSFREADARRIEAARQRQPFTNIHDLCLRAELEPRAREQ
LADAGVLRGLAGHRHRARWAVAGVEPQLPLFASLPATEETPINLPLPSVSEDLLNDYATL
GTTLGPHPLALLRDELKARRCRSSRELAMIEHGRPVSIAGLVIGRQRPQTASGVIFVTLE
DEFGMVNVVVWRDLAERQRRPLIQSQLLRVDGHLESASGVRHLIAGRLSDLTPLLTGLDV
RSRDFQ