Protein Info for Psest_1944 in Pseudomonas stutzeri RCH2

Updated annotation (from data): NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form
Rationale: Specifically important for utilizing L-Glutamic acid monopotassium salt monohydrate. Automated validation from mutant phenotype: the predicted function (GLUTAMATE-DEHYDROGENASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: NAD-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 35 to 178 (144 residues), 168.5 bits, E=3.2e-53
• PF21073: GDH_HM1
  • amino acids 344 to 403 (60 residues), 80.4 bits, see alignment (E = 2.9e-26)
• PF21076: GDH_ACT2
  • amino acids 407 to 496 (90 residues), 113 bits, 3.2e-36
• PF21079: GDH_HM2
  • amino acids 503 to 544 (42 residues), 52.9 bits, see alignment (E = 1.2e-17)
• PF21077: GDH_ACT3
  • amino acids 552 to 627 (76 residues), 93.7 bits, 1.9e-30
• PF21078: GDH_HM3
  • amino acids 646 to 709 (64 residues), 99.1 bits, see alignment (E = 5e-32)
• PF05088: Bac_GDH_CD
  • amino acids 731 to 1225 (495 residues), 899.2 bits, E=2.5e-274
• PF21074: GDH_C
  • amino acids 1270 to 1607 (338 residues), 439.1 bits, E=4.5e-135

Best Hits

Swiss-Prot: 76% identical to DHE2_PSEAE: NAD-specific glutamate dehydrogenase (gdhB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-alanine degradation II (to D-lactate) (3/3 steps found)
• L-glutamate degradation I (1/1 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (4/7 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Sequence Analysis Tools

See L0GID8 at UniProt or InterPro

Protein Sequence (1616 amino acids)

>Psest_1944 NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form (Pseudomonas stutzeri RCH2)
MAFFSAASKADFQQQLQTALAQHVDSKILPQLNLFAEQFFGIVALPELTERRMSDLVGST
LASWRLLERFEPSTPEVQVFNPDFEKHGWQSTHSVVEVLHPDMPFLVDSVRMELTRRGYS
IHTLQTSVLQVRRAADGSLLELLPKDERAADSHAESLIFVEIDRCASPSALRELEQSLLG
VLADVRQVVGDFAAMKGKAVELQGRLEQVNLSIDGDELDEIRDFMRWLADDHFTFLGYEE
FSVQEQADGGRIFYDESSLLGLSRTMRTGLSEEEQSLTAQSMSYLREPLLLSFAKAAMPS
RVHRPAYPDFVSVREFDEAGRVVRECRFLGLFTSSVYTQSVRRIPFIRRKVETVVQRANF
GSSAHLAKELVQVLEVLPRDELFQAPIDELFENAIAIVQIQERNRLRLFLRFDPYRRFCY
CLVYVPRDSYSTETRLKIQQVLQERLEASDCEFSTYFSESVLTRVQFILRLDPSRAVQVD
PVRLEQEVLQACRTWQDDYQSLVVERFGEAKGTHLLSEFPKGFPAGYRERFSPQSATVDM
QHVLDLSEERPLVMSFYQPITAEENRLHCKLYHLNTPLPLSDILPIMENLGLRVLGEFPF
RLRNRSGREYWIHDFAFTYAEGLEIDLLEINEALQDAFIHIYGGAAENDAFNRLVLTAGL
AWREVALLRAYARYLKQIRMGFDLGYIASALLNHTDIARELVRLFKMRFYLARKLGDEDL
ADKQQRLEQAILSALDDVAVLNEDRILRRYLALIQATLRTNFYQPDANGRPKPYFSFKLD
PRAIPEMPRPAPMYEIFVYSPRVEGVHLRGGKVARGGLRWSDREEDYRTEVLGLVKAQQV
KNAVIVPGGAKGGFVPRRLPTTGTRDDIQAEAIACYRIFISGLLDITDNLREGQVAPPAN
VLRYDGDDPYLVVAADKGTASFSDIANGIAAEYDFWLGDAFASGGSAGYDHKKMGITARG
AWVSVQRHFRERGINVQSDVISVIGIGDMAGDVFGNGLLMSETLQLVAAFNHLHIFIDPN
PDPARSFLERKRLFDLPRSSWTDYDASLISEGGGIFPRSAKRVQISPQMKERFAIEADQL
TPAELINALLKAPVDLLWNGGIGTYVKSSSESHAEVGDKANDAVRVNGAELRAKVVGEGG
NLGMTQLGRVEYALHGGSSNTDFIDNAGGVDCSDHEVNIKILLNEVVSAGDMTAKQRNQL
LFEMTDAVAELVLQNNYKQTQALSQAQHRSRERAAEYVRLINALEAAGQLDRALEFLPSD
EALAERASIGKGLTRPELSVLISYSKIELKKALLDSRVPDDDYLAREMDSAFPQQLAEHF
RAAMLQHRLKREIVATQIANDLVNNMGITFVQRLNEATGMSAANVAGAYVIVRDIFHLPH
WFRQIEALDHKVPAELQLSLMDELMRLGRRATRWFLRNRRSELDAARDVAHFGPRVAALG
LKLDELLQGSTRDHWQERYRRYTEAGVPELLARMVAGTNHLYTLLPILEAADETGQVPAQ
VAAAYFAVGGALELPWYLHQLTNMPVGNNWQALAREGFRDDLDSQQRSITVSVLQMENGA
ESISERVDAWLAQRPVPLARWRSMLAELRNASGNDYAIYAVASRELQGLAQSARHG