Protein Info for Psest_1910 in Pseudomonas stutzeri RCH2

Annotation: 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 PF06029: AlkA_N" amino acids 4 to 121 (118 residues), 128.4 bits, see alignment E=1.7e-41 PF00730: HhH-GPD" amino acids 127 to 251 (125 residues), 31.5 bits, see alignment E=1.9e-11

Best Hits

KEGG orthology group: K01247, DNA-3-methyladenine glycosylase II [EC: 3.2.2.21] (inferred from 86% identity to psa:PST_2427)

Predicted SEED Role

"DNA-3-methyladenine glycosylase II (EC 3.2.2.21)" in subsystem DNA Repair Base Excision (EC 3.2.2.21)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GM50 at UniProt or InterPro

Protein Sequence (294 amino acids)

>Psest_1910 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase (Pseudomonas stutzeri RCH2)
MTETLHLPYLQPWDWQQFHRHFALRLLPGVERLDADGYARTLRLGDARGWLSVRALTDRP
ALEMTLSDSLQHAAKPLVSQVRKMFDLDADPQAIAAHFTSDPTLGPLIAVQPGLRLPAAY
DPFEQAVRAVVGQQVTVKAAVTITRRLVERLGEPLQGAPSGLELLFPTAGAIADDPLENI
GMPARRAQALRRLAAAVADGALQLHVEDGADALVRKLCELPGIGPWTAEYIALRGFGVAD
AFPAADLGLLKAPLWGDGGITAKALTARAESWRPWRAYAAIHIWDNYAKGSKGG