Protein Info for Psest_1893 in Pseudomonas stutzeri RCH2
Annotation: glucokinase, proteobacterial type
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to GLK_PSEA8: Glucokinase (glk) from Pseudomonas aeruginosa (strain LESB58)
KEGG orthology group: K00845, glucokinase [EC: 2.7.1.2] (inferred from 89% identity to psa:PST_2443)Predicted SEED Role
"Glucokinase (EC 2.7.1.2)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis (EC 2.7.1.2)
MetaCyc Pathways
- Bifidobacterium shunt (15/15 steps found)
- homolactic fermentation (11/12 steps found)
- glycolysis III (from glucose) (10/11 steps found)
- heterolactic fermentation (15/18 steps found)
- glycogen degradation I (7/8 steps found)
- trehalose degradation II (cytosolic) (2/2 steps found)
- glucose and glucose-1-phosphate degradation (4/5 steps found)
- sucrose biosynthesis II (6/8 steps found)
- GDP-α-D-glucose biosynthesis (2/3 steps found)
- trehalose degradation V (2/3 steps found)
- UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
- glycogen degradation II (4/6 steps found)
- trehalose degradation I (low osmolarity) (1/2 steps found)
- chitin biosynthesis (6/9 steps found)
- sucrose degradation III (sucrose invertase) (2/4 steps found)
- UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
- trehalose degradation IV (1/3 steps found)
- 1,3-propanediol biosynthesis (engineered) (5/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Nucleotide sugars metabolism
- Starch and sucrose metabolism
- Streptomycin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.2
Use Curated BLAST to search for 2.7.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GM83 at UniProt or InterPro
Protein Sequence (320 amino acids)
>Psest_1893 glucokinase, proteobacterial type (Pseudomonas stutzeri RCH2) MSTALVGDIGGTNARFALWRDQRIEQIRVLPTADYASPELAIRAYLREVDQPLDALEAVC LACAGPVGGDLFRFTNNHWQLSREAFCRELGVKELLLINDFTAMALGMTRLHDGERITVC QGEPEPGRPRLVIGPGTGLGVAGLLPLSGGGWRALPGEGGHICLPIGSEREAAIWAHLHR SQGHVNAEAVLSGPGLLTLYRACCALDGQQVEFDSPAAITKAALAGDAYATAVLEQFCRW LGRIVGDNVLTLGARGGVYIVGGVVPRFAEMFLRSGFSEALREKGQMSRYFDRLPVWLVT APYPGLEGAGVALQPLLEGA