Protein Info for Psest_1875 in Pseudomonas stutzeri RCH2

Annotation: Adenylate cyclase, family 3 (some proteins contain HAMP domain)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 58 to 78 (21 residues), see Phobius details amino acids 85 to 104 (20 residues), see Phobius details amino acids 124 to 153 (30 residues), see Phobius details amino acids 160 to 179 (20 residues), see Phobius details amino acids 189 to 210 (22 residues), see Phobius details PF05230: MASE2" amino acids 56 to 145 (90 residues), 103.1 bits, see alignment E=6.9e-34 PF00211: Guanylate_cyc" amino acids 262 to 445 (184 residues), 110.5 bits, see alignment E=7.9e-36

Best Hits

KEGG orthology group: None (inferred from 91% identity to psa:PST_2461)

Predicted SEED Role

"Adenylate cyclase (EC 4.6.1.1)" in subsystem cAMP signaling in bacteria (EC 4.6.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.6.1.1

Use Curated BLAST to search for 4.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKA6 at UniProt or InterPro

Protein Sequence (500 amino acids)

>Psest_1875 Adenylate cyclase, family 3 (some proteins contain HAMP domain) (Pseudomonas stutzeri RCH2)
MPNALFSATAFAADGLRSRESARRLEKASTNYQGSYAYMTSTTVERRNGLATRPLREYYS
RVLAYLICAATLAAGTYTQYFSMDLLWMVPYALLYPHLAYHLSYRFKRDHPERTSQVLLC
LDALNAGAGVALLGFSIVPSLMFLLTLSFSALVIGSLRNLLMALICAAAGTGLILLAIPM
EYRGTTPPLVSLVSILFTTLYVCATAYFVHQQGLRLAIARKEIETEQAKAARLARNLAKY
LSPQVWESIFTGKRSVRLETQRKRLTVFFSDIKGFTELAEELEAEALTDLLNNYLNEMSK
IALKYGGTIDKFVGDCVMVFFGDPSTQGSKKDAVAAVSMAIAMRKHMKVLRQQWRAQGIT
KPMEIRMGINTGYCTVGNFGADTRMDYTIIGREVNLASRLESSAEASEILVSHETYSLIK
DVIMCRDKGQIPVKGFSRPVQIYQVVDFRRDLGATCSFVEHELPGFSMYLDTNGVQNFDK
ERVIQALQQAADKLRDKVIL