Protein Info for Psest_1830 in Pseudomonas stutzeri RCH2

Annotation: Periplasmic protein involved in polysaccharide export

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF02563: Poly_export" amino acids 72 to 156 (85 residues), 86 bits, see alignment E=2.4e-28 PF10531: SLBB" amino acids 247 to 296 (50 residues), 23 bits, see alignment 8.3e-09 PF18412: Wza_C" amino acids 337 to 354 (18 residues), 31.4 bits, see alignment (E = 1.9e-11)

Best Hits

KEGG orthology group: K01991, polysaccharide export outer membrane protein (inferred from 93% identity to psa:PST_2501)

Predicted SEED Role

"Polysaccharide export lipoprotein Wza" in subsystem Colanic acid biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GK54 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Psest_1830 Periplasmic protein involved in polysaccharide export (Pseudomonas stutzeri RCH2)
MKPFIALAFTSALALQGCAFAPGQYLDPDEFKDGAEAEHGTVQLIRITPEMLKGELASDK
GTSSKQELLNYKPSAYRIGANDLLYITVWDHPELTAPSGPQQQLDANGRLVRPDGTLFYP
YIGNVDAAGKTIEELRATIARRLTNYIDSPQVDVSILRFGSQRVVVSGAFNTAGEVPVTT
APMTITQAIGQAGVDTETADLTGLTLKRDGREYMLDMDSLNRPDSWLHQVYLKDGDQLHL
PYNDQRKIYVLGEVNLPQALSFKATSMNLMDAVGTAGGIRQETADGEALYVIRGAENMAT
EPAKVFQLNAKSPTAFALAKHFPLQPQDVVFVGPANITRWNRFISQLLPSANVIGIGAAA
DYNLGR