Protein Info for Psest_1825 in Pseudomonas stutzeri RCH2

Annotation: Predicted nucleoside-diphosphate sugar epimerases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 transmembrane" amino acids 24 to 46 (23 residues), see Phobius details amino acids 59 to 78 (20 residues), see Phobius details amino acids 90 to 111 (22 residues), see Phobius details amino acids 124 to 142 (19 residues), see Phobius details PF02719: Polysacc_synt_2" amino acids 306 to 612 (307 residues), 411.1 bits, see alignment E=6e-127 PF04321: RmlD_sub_bind" amino acids 306 to 435 (130 residues), 31.3 bits, see alignment E=2.8e-11 PF01370: Epimerase" amino acids 306 to 538 (233 residues), 54.6 bits, see alignment E=2.7e-18 PF01073: 3Beta_HSD" amino acids 307 to 439 (133 residues), 26.9 bits, see alignment E=6.3e-10 PF16363: GDP_Man_Dehyd" amino acids 307 to 434 (128 residues), 38.8 bits, see alignment E=2e-13

Best Hits

KEGG orthology group: None (inferred from 76% identity to pae:PA3141)

MetaCyc: 76% identical to UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (Pseudomonas aeruginosa)
RXN-13252 [EC: 4.2.1.135]

Predicted SEED Role

"nucleotide sugar epimerase/dehydratase WbpM"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GK49 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Psest_1825 Predicted nucleoside-diphosphate sugar epimerases (Pseudomonas stutzeri RCH2)
MFVLSLAEKLRARLVSLPRRYKRLIQLAADIVLVWVALWLAFVVRLGDSKNIEPLDGHAW
LFGIAPVIAIPFFIRFGMYRAVMRYFGNDALMAIAKAVTLSALLLSLAVYWNSDAPKLIP
RSMVFNYWWLSLVLIGGLRLVMRQYFMGDWFSPTTLTKFKGKEAGLPKVAVYGAGAAGNQ
LVAALRMGRAMRPVAFIDDDPNLHNRVIAGLRVYSAKHIGQLVEDTGAGEILLAIPSASR
GRRREILEMLERFPLHVRSVPGFMDLASGKVQVEDIQEVDIADLLGRDAVPPQQALFEKC
IRGKVVMVTGAGGSIGSELCRQILSNKPQALLLFEHSEFNLYSIHMELERLIERTSLPIR
LVPILGSIRNADRLLDVMRTWGVETIYHAAAYKHVPMVEHNVAEGVLNNVIGTLNTAQAA
VQAGVSNFVLISTDKAVRPTNVMGSTKRVAELVLQALSREPAPGLFGTAGSVHHVNKTRF
TMVRFGNVLGSSGSVIPRFYAQIRAGGPVTVTHPKITRYFMTIPEAAQLVIQAGSMGQGG
DVFVLDMGQPVKIAELAEKLIHLSGLSVRSEKCPHGDIAIEYTGLRPGEKLYEELLIGDN
VSPTEHPMIMRADEEYLDWDVLKDRLAKLLKAVESDDYPQVRQLLREVVSGYVPEGEIVD
WIYQQRRVEPE