Protein Info for Psest_1821 in Pseudomonas stutzeri RCH2
Annotation: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to MANC_ECOLI: Mannose-1-phosphate guanylyltransferase (manC) from Escherichia coli (strain K12)
KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 77% identity to pfs:PFLU3664)MetaCyc: 66% identical to mannose-1-phosphate guanylyltransferase (Escherichia coli K-12 substr. MG1655)
Mannose-1-phosphate guanylyltransferase. [EC: 2.7.7.13]
Predicted SEED Role
"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (10/11 steps found)
- GDP-mannose biosynthesis (4/4 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.13, 2.7.7.22
Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GKQ2 at UniProt or InterPro
Protein Sequence (469 amino acids)
>Psest_1821 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Pseudomonas stutzeri RCH2) MLLPVIIAGGSGSRLWPLSRQLNPKQFLALTDAQLSMLQSTIRRLDGLEAGLPLLICNEQ HRFLAAEQLRQLSMEQASILLEPVGRNTAPAIALAALQATQEGDDPILLVLAADHLIQDV DAFHSSIQAAMPFATNGKLVTFGIVPTNPETGYGYIEKGKELGEGGFAVNRFVEKPSLDI AEEYLASGEYFWNSGMFMFRASRYLDELERHQPSILAACRQALAAGTQDMHFVRVDAATF AACPEDSVDYAVMEKTNDAVMVPLDAGWSDVGSWTALWEASNKDAEGNVFKGDVLGHATR NSFVHADSRLVATLGVEDLVIVETKDAVLVAHKNQVQDVKKLVERIKADNRHEHLNHREV YRPWGVYDAIDSGHRYQVKRITVQPGAKLSVQMHHHRAEHWVVVSGTAKVTNGDKTYLVT ENQSTYIPVGQVHALENPGVIPLELIEVQSGSYLGEDDIVRFEDQYGRA