Protein Info for Psest_1819 in Pseudomonas stutzeri RCH2

Annotation: Nucleoside-diphosphate-sugar epimerases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01370: Epimerase
  • amino acids 9 to 232 (224 residues), 218.7 bits, E=8e-69
• PF16363: GDP_Man_Dehyd
  • amino acids 43 to 309 (267 residues), 75 bits, E=8.1e-25

Best Hits

Swiss-Prot: 71% identical to FCL_ECOLI: GDP-L-fucose synthase (fcl) from Escherichia coli (strain K12)

KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 77% identity to pfv:Psefu_1688)

MetaCyc: 71% identical to GDP-L-fucose synthase (Escherichia coli K-12 substr. MG1655)
GDP-L-fucose synthase. [EC: 1.1.1.271]

Predicted SEED Role

"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.271

Sequence Analysis Tools

See L0GLV4 at UniProt or InterPro

Protein Sequence (325 amino acids)

>Psest_1819 Nucleoside-diphosphate-sugar epimerases (Pseudomonas stutzeri RCH2)
MARDLNQTIFVAGHRGMVGSAIVRRLRALGYDNILTTGRDELNLLDQQAVHAWFQSHAIN
QVYLAAAKVGGIHANNTFPADFIYENLMIEANIIHAAHIHGVQKLLFLGSSCIYPKHAEQ
PMREESLLTATLEPTNEPYAIAKIAGIKLCESYNRQHVRDYRSVMPTNLYGPHDNFHPDN
SHVIPALLRRFHEAVQRGDKEVVIWGSGKAMREFLHVDDMAAASVHVMELDQAAYQAATQ
PMLSHINVGTGVDCTIRTLAETIASVTGFKGQLIFDSNKPDGAPRKLMDASRLKSLGWEA
SITLEDGLRSAYGWYVSNVEQARSQ