Protein Info for Psest_1818 in Pseudomonas stutzeri RCH2
Annotation: GDP-mannose 4,6-dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to GM4D_VIBCL: GDP-mannose 4,6-dehydratase (gmd) from Vibrio cholerae
KEGG orthology group: K01711, GDPmannose 4,6-dehydratase [EC: 4.2.1.47] (inferred from 89% identity to pfv:Psefu_1689)MetaCyc: 82% identical to GDP-mannose 4,6-dehydratase monomer (Escherichia coli O157:H7)
GDP-mannose 4,6-dehydratase. [EC: 4.2.1.47]
Predicted SEED Role
"GDP-mannose 4,6-dehydratase (EC 4.2.1.47)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 4.2.1.47)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (10/11 steps found)
- GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
- GDP-6-deoxy-D-talose biosynthesis (1/2 steps found)
- GDP-D-perosamine biosynthesis (1/2 steps found)
- GDP-D-rhamnose biosynthesis (1/2 steps found)
- GDP-L-colitose biosynthesis (3/5 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
- GDP-N-acetyl-α-D-perosamine biosynthesis (1/3 steps found)
- GDP-N-formyl-α-D-perosamine biosynthesis (1/3 steps found)
- GDP-mycosamine biosynthesis (1/3 steps found)
- superpathway of candicidin biosynthesis (4/11 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.2.1.47
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GM06 at UniProt or InterPro
Protein Sequence (372 amino acids)
>Psest_1818 GDP-mannose 4,6-dehydratase (Pseudomonas stutzeri RCH2) MRKALITGITGQDGSYLAELLLEKGYEVHGIKRRASLFNTQRVDHLYQDPHVNNRNFVLH YGDLSDSSNLTRIIQEVQPDEVYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRLLG LEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYACN GILFNHESPRRGETFVTRKITRGLANIAQGLEQCLYMGNLDALRDWGHAKDYVRMQWMML QQEQPEDFVIATGVQYSVREFIRWSAAELGITLKFEGQGVEELAIIEAIEGEKAPALKVG DVVVRVDPRYFRPAEVETLLGDPTKAKDKLGWVPEITVQEMCAEMVREDLTAARRHALLK QHGHDIPVPMEN