Protein Info for Psest_1744 in Pseudomonas stutzeri RCH2

Annotation: Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 PF00072: Response_reg" amino acids 5 to 109 (105 residues), 79.2 bits, see alignment E=2.6e-26 PF01339: CheB_methylest" amino acids 188 to 363 (176 residues), 210.5 bits, see alignment E=1.5e-66

Best Hits

Swiss-Prot: 84% identical to CHEB1_PSEPK: Protein-glutamate methylesterase/protein-glutamine glutaminase of group 1 operon (cheB1) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03412, two-component system, chemotaxis family, response regulator CheB [EC: 3.1.1.61] (inferred from 91% identity to psa:PST_2565)

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHR6 at UniProt or InterPro

Protein Sequence (369 amino acids)

>Psest_1744 Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain (Pseudomonas stutzeri RCH2)
MAVKVLVVDDSGFFRRRVSEILSSDSNIQVVGTATNGREAIDQVLALKPDVITMDYEMPM
MDGITAVRQIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDYLPKNFEDISRNPEKVKQ
MLCEKVHTISRSNRRYSTFCTSSVNPPPAPSRGAVPSASTNTLRSTGAAPTAPAARSPAP
KRKAYKLVAIGTSTGGPVALQRVLTQLPANFPAPIVLIQHMPAAFTKAFADRLDKLCRIR
VKEAEDGDVLRPGLALLAPGGKQMMIDSRGAVRILPGDDRLNYKPCVDVTFGSAAKSFHD
KVLAVVLTGMGADGREGARLLKQSGSQVWAQDEASCVIYGMPMAVVKANLSDAVYGLDEI
GRNLAEACI