Protein Info for Psest_1743 in Pseudomonas stutzeri RCH2

Annotation: Chemotaxis protein histidine kinase and related kinases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 transmembrane" amino acids 585 to 601 (17 residues), see Phobius details PF01627: Hpt" amino acids 8 to 99 (92 residues), 58.1 bits, see alignment E=1.8e-19 PF02895: H-kinase_dim" amino acids 338 to 397 (60 residues), 61.7 bits, see alignment 1.5e-20 PF02518: HATPase_c" amino acids 445 to 584 (140 residues), 64.9 bits, see alignment E=1.8e-21 PF01584: CheW" amino acids 591 to 718 (128 residues), 98.3 bits, see alignment E=5.9e-32

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 85% identity to psa:PST_2566)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKH1 at UniProt or InterPro

Protein Sequence (723 amino acids)

>Psest_1743 Chemotaxis protein histidine kinase and related kinases (Pseudomonas stutzeri RCH2)
MSFDADEEILQDFLVEAGEILELLSEQLVELESSPDDMALLNAIFRGFHTVKGGAGFLQL
HELVECCHIAENVFDILRKGERRVDSELMDVVLQALDSVNEMFGQVRERVEPTPASRELL
AALARLAEPASADEPAVPMEPAPVAEPQVEAVDAEFEQMLAAVQSSDSEPAAAQVSGGGD
EITDDEFESLLDQLHGKGQFSVSAASAAPEPEVVVAPASDEITDDEFEALLDQLHGKGKF
EPVGSTSTEPATPAAAAPASNTSASDEITDDEFEALLDQLHGKGKFEPAVAEVAPAVPSK
PEPKPEAARAAPAKAAPAAAVSKPAAVVAEKAPAEAETTVRVDTARLDEIMNMVGELVLV
RNRLVRLGSNSGDEAMAKAVSNLDVVTADLQSAVMKTRMQPIKKVFGRFPRLVRDLARSL
KKEINLELVGEETDLDKNLVEALADPLVHLVRNAVDHGIEMPEEREAAGKPRTGRVVLSA
EQEGDHILLIISDDGKGMDANVLRAKAVEKGMLEKDAADRLSDLECYNLIFAPGFSTKTE
ISDVSGRGVGMDVVKTKISQLNGTVNVFSTKGQGSRVVIKVPLTLAIMPTLMVMLGNQAF
AFPLVNVNEIFHLDLSRTNVVDGQEVVIVRDKALPLFYLKRWLIQSAAEEEQREGHVVIL
SVGNQSIGFVVDQLVGQEEVVIKPLGKMLQGTPGMSGATITGDGRIALILDVPSMLKRYA
RRI