Protein Info for Psest_1740 in Pseudomonas stutzeri RCH2

Annotation: RNA polymerase sigma factor, FliA/WhiG family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 12 to 235 (224 residues), 116.7 bits, see alignment E=8e-38 TIGR02479: RNA polymerase sigma factor, FliA/WhiG family" amino acids 15 to 235 (221 residues), 272.7 bits, see alignment E=2.6e-85 PF04542: Sigma70_r2" amino acids 15 to 87 (73 residues), 70.2 bits, see alignment E=2.1e-23 PF04539: Sigma70_r3" amino acids 97 to 164 (68 residues), 42.3 bits, see alignment E=1.3e-14 PF08281: Sigma70_r4_2" amino acids 179 to 223 (45 residues), 34.7 bits, see alignment 2.2e-12 PF04545: Sigma70_r4" amino acids 184 to 233 (50 residues), 65.1 bits, see alignment 6.5e-22

Best Hits

Swiss-Prot: 82% identical to FLIA_PSEAE: RNA polymerase sigma factor FliA (fliA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02405, RNA polymerase sigma factor for flagellar operon FliA (inferred from 98% identity to psa:PST_2569)

MetaCyc: 53% identical to RNA polymerase sigma factor FliA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor for flagellar operon" in subsystem Flagellar motility or Flagellum or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLT3 at UniProt or InterPro

Protein Sequence (241 amino acids)

>Psest_1740 RNA polymerase sigma factor, FliA/WhiG family (Pseudomonas stutzeri RCH2)
MYYSKAQAKDAQYRLIDQYAPLVKRIAYHLLARLPASVQVDDLIQAGMIGLLEAAKKYDA
GKGASFETYAGIRIRGTMLDEVRKGDWAPRSVHRNSRMVSEAIRTIEAKTGRDAKDHEVA
AELKLSLDEYYGILGDTLGSRLFSFDDLLQEGEHGELQEDAASLHPEPFRDLEDERFQQA
LVDAIANLPEREKLVLSLYYDEELNLKEIGQVLGVSESRVSQLHSQCAARLRARLGEWRA
N