Protein Info for Psest_1719 in Pseudomonas stutzeri RCH2

Annotation: flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 transmembrane" amino acids 39 to 59 (21 residues), see Phobius details amino acids 489 to 511 (23 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 30 to 593 (564 residues), 293.1 bits, see alignment E=2.6e-91 PF01514: YscJ_FliF" amino acids 60 to 236 (177 residues), 215.5 bits, see alignment E=5.2e-68 PF08345: YscJ_FliF_C" amino acids 269 to 466 (198 residues), 144.6 bits, see alignment E=3.2e-46

Best Hits

Swiss-Prot: 77% identical to FLIF_PSEAE: Flagellar M-ring protein (fliF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 96% identity to psa:PST_2590)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHP3 at UniProt or InterPro

Protein Sequence (594 amino acids)

>Psest_1719 flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF) (Pseudomonas stutzeri RCH2)
MAEALSNVPVPATTENAKKPLLGLSFLENLSDMSMLRQVGLLVGLAASVAIGFAVVLWSQ
QPDYRPLLGSLAGMDANQVMETLAAADIAYTVEPNSGALLVKANDLARARLKLASAGIAP
ADSNIGFEILDKDQGLGTSQFMEATRYRRGLEGELGRTISSLNNVKGARVHLAIPKSSVF
VRDERKPSASVLVELYPGRALEPSQVMAIINLVATSVPELNKSQITVVDQKGNLLSDQQE
LTELSMAGKQFDYSRRMESLYTQRVHNILQPVLGSGRYKAEVSADVDFSAVESTSETFNP
DQPALRSEQSVNEQRQSSLPPQGVPGALSNQPPGPAAAPEQANQAAAAAGAVAPGQPLLD
ANGQQIMDPATGQPMLAPFPADKREQATRNYELDRSISYTKQQHGRLRRLSVAVVVDDQM
TLNAAGEMVRVPWTADDLARFTRLVQDSVGFDASRGDSVSVINTAFVADSFGETFEEIPF
YSQPWFWDVVKQVLGVLFILVLVFGVLRPVLKSLTNPSSGKELQVANGPGDLGDEEGLES
GLSNDRVSLSGPQNILLPSPSEGYEAQLNAIKNLVADDPGRVAQVVKEWINADE