Protein Info for Psest_1681 in Pseudomonas stutzeri RCH2

Annotation: haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 PF00702: Hydrolase" amino acids 5 to 180 (176 residues), 99.2 bits, see alignment E=1e-31 PF13419: HAD_2" amino acids 7 to 186 (180 residues), 117.3 bits, see alignment E=2.2e-37 PF12710: HAD" amino acids 8 to 177 (170 residues), 33.8 bits, see alignment E=1.2e-11 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 103 to 180 (78 residues), 37.8 bits, see alignment E=3.6e-13 PF13242: Hydrolase_like" amino acids 142 to 211 (70 residues), 50.5 bits, see alignment E=3.9e-17

Best Hits

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 79% identity to psa:PST_2629)

Predicted SEED Role

"Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJP7 at UniProt or InterPro

Protein Sequence (228 amino acids)

>Psest_1681 haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E (Pseudomonas stutzeri RCH2)
MSKYQVLIFDWDGTLVDSIGRIVESIHVAARSCGLPQVDDDLVKGIIGLALPEAITELYP
ERGDIGLIDDFRHFYSEHYLALEAQPSALYPGVARALSEFRDSGYLLAVATGKGRRGLDR
VLAGQGWEEFFDITRCADETASKPDPLMIQEILAHCGARPEQALMVGDSIFDLQMARRAG
VDSVAVAYGAQPLHILRRHEPRAVINHFSELGSWLRSAAGTEVHVNVG