Protein Info for Psest_1679 in Pseudomonas stutzeri RCH2

Annotation: ribonuclease, Rne/Rng family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1060 TIGR00757: ribonuclease, Rne/Rng family" amino acids 13 to 422 (410 residues), 557.3 bits, see alignment E=1.1e-171 PF00575: S1" amino acids 36 to 115 (80 residues), 49.2 bits, see alignment 8.4e-17 PF10150: RNase_E_G" amino acids 119 to 388 (270 residues), 340.3 bits, see alignment E=1.3e-105 PF20833: RNase_E_G_Thio" amino acids 400 to 484 (85 residues), 110.9 bits, see alignment E=4e-36

Best Hits

KEGG orthology group: K08300, ribonuclease E [EC: 3.1.26.12] (inferred from 83% identity to psa:PST_2631)

Predicted SEED Role

"Ribonuclease E (EC 3.1.26.12)" in subsystem RNA processing and degradation, bacterial or Ribosome biogenesis bacterial (EC 3.1.26.12)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.26.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLM3 at UniProt or InterPro

Protein Sequence (1060 amino acids)

>Psest_1679 ribonuclease, Rne/Rng family (Pseudomonas stutzeri RCH2)
MKRMLINATQPEELRVALVDGQRLFDLDIESGAREQKKANIYKGKITRVEPSLEAAFVDF
GAERHGFLPLKEISREYFSKSPEGRVNIKDVLREGQEVIVQVEKEERGNKGAALTTFISL
AGRYLVLMPNNPRAGGISRRIEGEERNELREALNGLDIPADMGLIVRTAGLGRSSEELQC
DLDYLLQLWTAVKDASKDRAAPFLIYQESNVIIRAIRDYLRQDIGEVLIDSVEAQDEALS
FIQQVMPQYASKIKLYEDSVPLFNRFQIESQIETAFQREVKLPSGGSIVIDPTEALVSID
INSARATKGGDIEETALQTNLEAAEEIARQLRLRDIGGLIVIDFIDMTPAKNQRAVEEKM
REALEADRARIQVGRISRFGLLEMSRQRLRPSLGETSGIVCPRCNGQGIIRDVESLSLAI
LRLIEEEALKDRTAEVRARVPFQVAAFLLNEKRNAITKIELRTRARIFILPDDHLETPHF
EVQRLRDDSPEILAGQASYEMSQTEAEEAQPVSSTRTLVRQEAAVKTAPQRTAPAPAAAA
APAEAPAAAPAQEPSLFKGLIKSLVGLFAGEAKEPQAAAEVEKKPASPRPQRNDERRSGR
QQNRRRDSRGGRDEERKPREERQPREERQPREERQPRDDRQPREERQPRPPREERKPREQ
VEATEAQPRRERAPREERKPREERKRELRAPIDEAPVTAAEEEQVERQPRAPREERKPRA
EQQAVAAADELLQQTEDANEAEDAQDTNEASEGNDGERPRRRSRGQRRRSNRRERQRDAN
GNEIDEVDESNAPVKTEEIAVAATAAALAANTTDTEAAPQVKLADDAVIQTSTEGVATIV
EQAAVAEVTDERTAAEEPAVVEAAVEAPVQPTEVAAGEVAPSEVPAVQTDVTPQPASEPV
VEKTEAAPAAAPALTPSGRAPNDPREVRRRQREAERLAKEAAEAEAKAAAAQPLASPEIV
ASEPAEETQTEAIAQPVASDAQPEQVVESAPAAEQLASEEPSVSPTVEPTQQAQTPVTEP
VAEVVEEKPEAPVSEEAPQTAPEEKAAEGDEPDNREKPQG