Protein Info for HP15_1596 in Marinobacter adhaerens HP15

Annotation: D-amino acid dehydrogenase 2 small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF01266: DAO" amino acids 3 to 399 (397 residues), 255.3 bits, see alignment E=5.1e-79 PF00890: FAD_binding_2" amino acids 4 to 93 (90 residues), 30.5 bits, see alignment E=9.4e-11 PF13450: NAD_binding_8" amino acids 6 to 36 (31 residues), 35 bits, see alignment (E = 5.8e-12)

Best Hits

Swiss-Prot: 63% identical to DADA3_RHILO: D-amino acid dehydrogenase 3 (dadA3) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 76% identity to reh:H16_B0508)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PM32 at UniProt or InterPro

Protein Sequence (406 amino acids)

>HP15_1596 D-amino acid dehydrogenase 2 small subunit (Marinobacter adhaerens HP15)
MKRIAVIGGGITGITTAYTLAKRGLDVTVYEKHRYAAMETSFANGGQLSASNAEVWNNWQ
TVMKGIKWMSRRDAPLLVNPKPSWHKLSWFAEFIAAIPQYEKNTTETARLAIAARDHLFA
WAQEEGIDFDLKKQGILHIYRDKAGFDHAEKVSRLLAAGGLERRSVTPEEMKSIEPTLAG
NYYGGFFTESDSTGDIHKFTNGLADAIKRLGVKTCYGHTVTELSADERNAWVTAHDGTEQ
SRDTFDGVVICAGVGSRGLAKKLGDRVNIYPVKGYSITVELDDEASQKAAPTVSLLDDAT
KIVTSRLGDGRFRVAGTAEFSGYNRDIKDDRIRPLTRWVEQCFPGVCTRKVVPWAGLRPM
LPNMMPRVGPGRLPTVFYNTGHGHLGWTLSAITAELVAESVTGDSR