Protein Info for PGA1_c16580 in Phaeobacter inhibens DSM 17395

Annotation: CheR and CheB-like domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1083 PF01339: CheB_methylest" amino acids 12 to 189 (178 residues), 183.6 bits, see alignment E=1.3e-57 PF03705: CheR_N" amino acids 216 to 263 (48 residues), 41.8 bits, see alignment (E = 3.1e-14) PF01739: CheR" amino acids 277 to 461 (185 residues), 179.4 bits, see alignment E=2.6e-56 PF13596: PAS_10" amino acids 731 to 830 (100 residues), 33 bits, see alignment E=3.3e-11 PF00989: PAS" amino acids 974 to 1077 (104 residues), 29.7 bits, see alignment E=2.6e-10 TIGR00229: PAS domain S-box protein" amino acids 976 to 1080 (105 residues), 33.9 bits, see alignment E=1.5e-12 PF13426: PAS_9" amino acids 977 to 1077 (101 residues), 30.8 bits, see alignment E=1.3e-10 PF08448: PAS_4" amino acids 980 to 1081 (102 residues), 25.6 bits, see alignment E=5.4e-09 PF08447: PAS_3" amino acids 989 to 1074 (86 residues), 62.4 bits, see alignment E=1.7e-20

Best Hits

KEGG orthology group: K13924, two-component system, chemotaxis family, CheB/CheR fusion protein [EC: 2.1.1.80 3.1.1.61] (inferred from 64% identity to rde:RD1_3107)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80, 3.1.1.61

Use Curated BLAST to search for 2.1.1.80 or 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EX30 at UniProt or InterPro

Protein Sequence (1083 amino acids)

>PGA1_c16580 CheR and CheB-like domain-containing protein (Phaeobacter inhibens DSM 17395)
MTTQTTQDLTIIGIGSSAGGLEAIRELVATLPVDVPAAYVVVQHMSPHHKSLMTELVARQ
TQLKVGTIQNGTIPEANVVYITPPKSDVIFSDGRLHLLQDPNAEPGTPRPSVDRFLVSLA
EQNGDNSMAIILSGTGSDGAYGVQAIREAGGITIAQDAESAKYDGMPVAAVQTGCVDLVL
RPQDIGANLQKILSSPRDFTSFRHENSLRDSPVSDLLQILLARTRVDFRDYKQTTISRRI
ERRMIALGIETQEEYTQFCRNNPHAVDALFKDLLISVTRFFRDREEFELLKALLPQLIKE
RGPGPLRIWVAGCATGEEAYSIAMVVSEALGNPTNQLKSRVQIFATDIDKDALKTARKGV
YSLAALNDIPSELADKYLIRQTDGVRVVDNLRNAVLFSDHNVCQDPPFQKVDLICCRNLL
IYFGNPLQQKVMSRFHYAMSPHGLLFLGTAESVAGSDELFVQDRSASHVYRKRSLRAHQA
QQPYNMPSVPMMAQRPTIKPPAQPGPSTDRQLFEALAQSLGKNSLLVTEDFSIARVYGSV
SQFIEVNETSSLRMHLDLLRSPLREEARSLITIALKNGAHKGGVRHLLAENDDEEIRLDV
YPIIAKDISERAALVVFTPVTVDRSRMNEAPIVGEEGDATFERIRNLENEVATTREALQQ
TIEELETSNEELQSLNEELQSTNEELQATNEELETSNEELQSTNEELITVNEELQVTASE
LSGRTGELISVLESTPLAIVVADSALQITQATLAATRMFNIRRPISSPHISQCALPEGFP
ILAPICSEALRLGQTVTEEFTSKGTRIRLTCSPYFDVNGQILGVTMVVSEFPGMAHEMEM
ILDNSNLLLLNRTRSGEILRISSAYAELLGLSRDKALSKNLFALASDAGADEQMLREDAK
VLDSKDGFDNELLQVSLPGGEGDRWISMDRHRFPHHITEEPTVFTIGTDVTETVEARERA
EELLMQAELVQDMVGIGFWSIDADGQTPYWSPKVYEIHGVKPEDYDPEIDSAIAFFHPDD
REDVQRIVSEAIETGNGFSFIKRLIRPDGVEIQVESQGMAIRDDNGDVTRLVGIFREAQE
TDS