Protein Info for Psest_1661 in Pseudomonas stutzeri RCH2

Annotation: Membrane-associated lipoprotein involved in thiamine biosynthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF02424: ApbE" amino acids 44 to 324 (281 residues), 281.8 bits, see alignment E=3.1e-88

Best Hits

KEGG orthology group: K03734, thiamine biosynthesis lipoprotein (inferred from 87% identity to psa:PST_2649)

Predicted SEED Role

"Thiamin biosynthesis lipoprotein ApbE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJM7 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Psest_1661 Membrane-associated lipoprotein involved in thiamine biosynthesis (Pseudomonas stutzeri RCH2)
MIRASLRPVFAVVYLQPVIAVALATALTGCLFQDKVEVFTGPTMGSTYAVKYVRGGDGPS
KEELHGEVEVILGDVDKQLSTYRNDSDVERFNALPAGSCEPMPEMVRELVAAGDQLSADS
EGAFDLTLEPLLNLWGFGPQGRGERVPSTEDIEAAKALTGHRHLRIEGDRLCKAAAVQLD
FNSIAAGYAVDRVVDRLKALGVQSYLVEITGELKAEGRKPDGSPWRIAIEAPRDDQRVAQ
KIVELDGLGVSTSGDYRNYFEQDGRRYSHTLDPQSGRPIEHHLAAVTVIDRSTLRADGLS
TALMVLGPERGLALAERNAIAAFFVVREGQGFATMSTRAFDELFGAGVEQ