Protein Info for Psest_1660 in Pseudomonas stutzeri RCH2

Annotation: NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 signal peptide" amino acids 5 to 5 (1 residues), see Phobius details amino acids 26 to 28 (3 residues), see Phobius details transmembrane" amino acids 6 to 25 (20 residues), see Phobius details TIGR01941: NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit" amino acids 5 to 407 (403 residues), 746.8 bits, see alignment E=2.9e-229 PF00111: Fer2" amino acids 39 to 115 (77 residues), 45 bits, see alignment E=1.3e-15 PF00970: FAD_binding_6" amino acids 204 to 266 (63 residues), 38.5 bits, see alignment E=1.8e-13 PF00175: NAD_binding_1" amino acids 277 to 385 (109 residues), 59.8 bits, see alignment E=5.7e-20

Best Hits

Swiss-Prot: 87% identical to NQRF_PSEA7: Na(+)-translocating NADH-quinone reductase subunit F (nqrF) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K00351, Na+-transporting NADH:ubiquinone oxidoreductase subunit F [EC: 1.6.5.-] (inferred from 98% identity to psa:PST_2650)

MetaCyc: 69% identical to Na(+)-translocating NADH-quinone reductase subunit F (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit F (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLD9 at UniProt or InterPro

Protein Sequence (407 amino acids)

>Psest_1660 NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit (Pseudomonas stutzeri RCH2)
MSYEIFLAIGMFTAIVLALVVIILAARAKLVSSGDVSIEINGERTITVPAGGKLLQTLAS
NNIFLSSACGGGGTCAQCKCIVESGGGEMLPTEESHFTRREAGEGWRLSCQTPVKADMKI
EVPEEVFGVKKWECTVESNPNVATFIKELTLKLPEGENVDFRAGGYVQLECPPHEVRYKD
FDIQEEYRGDWDKFDQWKYVSKCDETVIRAYSMANYPEERGLVKFNIRIASPPPGRDDIP
PGKMSSYVFSLKPGDKITVYGPFGEFFAKDTDNEMVFIGGGAGMAPMRSHIFDQLKRLKS
TRKMSFWYGARSMREAFYVEEYDQLQAENPNFQWHLALSDPLPEDNWEGPTGFIHNVLYE
NYLKDHPAPEDCEFYMCGPPMMNASVIKMLTDLGVEPENILLDDFGG