Protein Info for Psest_1659 in Pseudomonas stutzeri RCH2

Annotation: NADH:ubiquinone oxidoreductase, Na(+)-translocating, E subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 38 to 59 (22 residues), see Phobius details amino acids 79 to 98 (20 residues), see Phobius details amino acids 110 to 132 (23 residues), see Phobius details amino acids 144 to 164 (21 residues), see Phobius details amino acids 176 to 198 (23 residues), see Phobius details TIGR01940: NADH:ubiquinone oxidoreductase, Na(+)-translocating, E subunit" amino acids 2 to 201 (200 residues), 352.5 bits, see alignment E=5.2e-110 PF02508: Rnf-Nqr" amino acids 4 to 200 (197 residues), 190.7 bits, see alignment E=1.1e-60

Best Hits

Swiss-Prot: 98% identical to NQRE_PSEU5: Na(+)-translocating NADH-quinone reductase subunit E (nqrE) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00350, Na+-transporting NADH:ubiquinone oxidoreductase subunit E [EC: 1.6.5.-] (inferred from 98% identity to psa:PST_2651)

MetaCyc: 71% identical to Na(+)-translocating NADH-quinone reductase subunit E (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit E (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLK3 at UniProt or InterPro

Protein Sequence (202 amino acids)

>Psest_1659 NADH:ubiquinone oxidoreductase, Na(+)-translocating, E subunit (Pseudomonas stutzeri RCH2)
MEHYLSLFVRAVFIENMALAFFLGMCTFIAISKKVETAIGLGIAVIVVQAITVPANNLIY
TYLLKSGALAWAGLPEVDLSFLGLLSYIGVIAAIVQILEMTLDKYVPSLYNALGVFLPLI
TVNCAIMGGTLFMVERDYNLGESVVYGVGSGLSWALAIALLAGIREKLKYSDVPDGLQGL
GITFITIGLMSLGFMSFSGVQL