Protein Info for Psest_1640 in Pseudomonas stutzeri RCH2

Annotation: (p)ppGpp synthetase, RelA/SpoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 PF13328: HD_4" amino acids 40 to 202 (163 residues), 152 bits, see alignment E=2.8e-48 TIGR00691: RelA/SpoT family protein" amino acids 69 to 743 (675 residues), 631.8 bits, see alignment E=7.4e-194 PF04607: RelA_SpoT" amino acids 262 to 370 (109 residues), 148 bits, see alignment E=3.1e-47 PF02824: TGS" amino acids 416 to 475 (60 residues), 83.3 bits, see alignment 2.5e-27 PF19296: RelA_AH_RIS" amino acids 494 to 643 (150 residues), 26.7 bits, see alignment E=1.4e-09 PF13291: ACT_4" amino acids 665 to 743 (79 residues), 69 bits, see alignment E=1.2e-22

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 99% identity to psa:PST_2669)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLB8 at UniProt or InterPro

Protein Sequence (747 amino acids)

>Psest_1640 (p)ppGpp synthetase, RelA/SpoT family (Pseudomonas stutzeri RCH2)
MVQVRALQPINTDGSINLDGWLDHVLGMDPALDRAALKEACEFAREAEQQANAAQNLWSE
GTSSYQIGLEIAEILADLKLDQDSLVAAVIYRGVREGKIQLADVNQRFGSVVAKLIEGVL
RMAAISASLNPRESLVLGSQAQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKLADDE
KRQRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPEQYKQIAQLLHERRLDREQYI
QNVVQQLKDELTATGIQPDIDGRAKHIYSIWRKMQKKGLQFSQIYDVRAVRVLVPEVRDC
YTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAEL
GVCAHWRYKGTDVNSGSDHYEEKIAWLRQVLEWHEELGDIGGLADQLRVDIEPDRVYVFT
PDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKVNGRIVPLNYSLQTGEQVEIITSKHGS
PSRDWLNPNLGYITTSRSRAKIVHWFKLQARDQNVAAGKALLERELGRLDLPPVDFDKLA
EKANLKNAEDMFAALGAGDLRLAHLVNMAQQLVEPERGYDQLELIPRRSAPYKPGKRGDV
QIQGVGNLLTQMAGCCQPLPGDPIVGYITQGRGVSIHRQDCPSVLQLAGREPERIIQVSW
GPVPEKTYPVEIMIRAYDRAGLLRDISQMLLNERINVLSMNTRSNKEDSTAQMTLTIEIP
GLNALGRLLSRISQLPNIIEAKRQRAT