Protein Info for Psest_1635 in Pseudomonas stutzeri RCH2

Annotation: Signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 917 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 167 to 189 (23 residues), see Phobius details PF09984: sCache_4" amino acids 36 to 167 (132 residues), 60.3 bits, see alignment E=5.8e-20 PF00672: HAMP" amino acids 189 to 238 (50 residues), 44.4 bits, see alignment 5.2e-15 PF00512: HisKA" amino acids 284 to 348 (65 residues), 86.6 bits, see alignment 2.8e-28 PF02518: HATPase_c" amino acids 395 to 510 (116 residues), 98.1 bits, see alignment E=1.3e-31 PF00072: Response_reg" amino acids 670 to 784 (115 residues), 97.1 bits, see alignment E=2.3e-31 PF01627: Hpt" amino acids 830 to 911 (82 residues), 58.7 bits, see alignment E=1.7e-19

Best Hits

Swiss-Prot: 72% identical to GACS_PSEPH: Sensor histidine kinase GacS (gacS) from Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CHA0)

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 94% identity to psa:PST_2674)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLB2 at UniProt or InterPro

Protein Sequence (917 amino acids)

>Psest_1635 Signal transduction histidine kinase (Pseudomonas stutzeri RCH2)
MLKDLGIRGRVLMLTLLPSTLLAVVLGAYFTWMQLSEMRHQLDERGQLIAEQLAPLAAPA
MNLGYDKRLQRILTQVLDQADVRAVTILDPERVQRVHAGPRMLTPPPPGDPEGLTRVSSM
DHTRILMPVLSRHLNLADEARGPDEKLIGWLELELSHHNTLLRGYRSLLTSLFLVSAGLI
ITALLALRMSRAISLPLQRVKAAVAQLKDGHLETRLPPLGSHEMDELASGINRMAEALLS
AREELQQSIDQATEDVQQNLETIEIQNIELDLARKEALEASRIKSEFLANMSHEIRTPLN
GILGFTQLLQKSDLTPRQQDYLGTIEKSADSLLGIINEILDFSKIEAGKLVLDSIPFNLR
DLIEDTLTILAPAAHTKQLELVSLVYRDTPLSLVGDPLRLKQVLTNLISNAIKFTNEGTI
AVRAMVEDDNADRAQLRISVQDTGIGLTDQDLRALFQAFSQADNSMSRQPGGTGLGLVIS
KRLIEQMGGEIGVDSIPDEGSEFWISLSLPKARDDVEDLPHPALQGRRIALLEQHPLARQ
ALQHQLESLGLEVHTFDTLDQMQAAIGAQRQSSQLIDLAVLGVTAREIQPEALSRRLMEI
EALGCKCVVLCPTTEQGHYHDTLPEVHSYTQLQGKPACTRKLQRVLVELVRPNQTYAAPV
AAAPSRAARVLCVDDNPANLLLVKTLLGDMGAEVVAVDNGPAALDAVKQHSFDLVLMDVQ
MPGMDGRQTTEAIRHWEHTSGSTPLPIIALTAHALANEKRSLLQSGMDDYLTKPISDRQL
AQVVLKWTGLVLRSQPQHSEPSSQPELSLKVLDKEEGLRLAAGKADLADDMLGMLLQSLE
GDRRTIREARQSGDRQALIERVHRLHGATRYCGVPQLRNACQQSETLLKQNHPDSETALD
ELDAAIDRLEQVVQVDD