Protein Info for Psest_1620 in Pseudomonas stutzeri RCH2

Annotation: Phosphate-selective porin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07396: Porin_O_P" amino acids 53 to 429 (377 residues), 267.6 bits, see alignment E=1.1e-83

Best Hits

Swiss-Prot: 54% identical to PORO_PSEAE: Porin O (oprO) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07221, outer membrane porin (inferred from 96% identity to psa:PST_2688)

Predicted SEED Role

"Pyrophosphate-specific outer membrane porin OprO" in subsystem Phosphate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL93 at UniProt or InterPro

Protein Sequence (429 amino acids)

>Psest_1620 Phosphate-selective porin (Pseudomonas stutzeri RCH2)
MIRKHFAGFAASALALAVSAQAFAGTVTTDGADLVIKTKGGLEVGTTDKEFSFKINGRLQ
ADADSFDGFYTQNGERADETYFRRARLEISGVAFTDWGYTFHRNFGNDSSDWQELAIHYN
GWEPVQVSIGRIIPTFGLEEAVSSKWITAIERSAIYDLAPWLNDHEDGEGIRVRTTVGMF
HGEAGAYRQDGGDGVDMLEDEDGANNTSFVLRGVVAPIVEKDQVLHLGASFGQRDLEEGY
LERIRPRLSVRGTTEDSANGNRATFGGTRTDGTDQAWGLEAAYMIGPFSVQGEYLTRTVD
AMEGFEDLEATGYNVQLAYTLTGESRSYKLDGGKFDKIKPENKQIGAWEVFYRFDDITVD
ETGVAPSGYVGALDSAEAGAKTHTIGLNWYANESVKLSANYLKTSVDDVVNANGDDDGDA
ISLRAQYVF