Protein Info for Psest_1619 in Pseudomonas stutzeri RCH2

Annotation: Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 PF00899: ThiF" amino acids 14 to 256 (243 residues), 139.8 bits, see alignment E=7.6e-45 PF02737: 3HCDH_N" amino acids 27 to 122 (96 residues), 24.3 bits, see alignment E=2.8e-09

Best Hits

Swiss-Prot: 59% identical to TCDA_ECOLI: tRNA threonylcarbamoyladenosine dehydratase (tcdA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to psa:PST_2689)

MetaCyc: 59% identical to tRNA threonylcarbamoyladenosine dehydratase (Escherichia coli K-12 substr. MG1655)
RXN0-7115

Predicted SEED Role

"CsdL (EC-YgdL) protein of the HesA/MoeB/ThiF family, part of the CsdA-E-L sulfur transfer pathway"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLG6 at UniProt or InterPro

Protein Sequence (269 amino acids)

>Psest_1619 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1 (Pseudomonas stutzeri RCH2)
MSIDDQRFGGIARLYGRVGLERLTAAHVAVVGIGGVGSWAAEALARSGVGEISLFDLDDV
CITNTNRQIHALDGAVGKAKVDEMAARIRAINPACQVHAVADFVTRETMAEYITEDMDCV
IDCIDSVSAKAALIAWCKRRKIQIITTGGAGGQVDPTQIQVTDLNKTFNDPLAAKVRSLL
RREYNFSRTPGRTYSVPCVFSTEQLRYPKPDGGVCQSKSFVGEGVKLDCAGGFGAVTMVT
ASFGMVAAARAVDKLVAGARRPSERNVRS