Protein Info for Psest_1618 in Pseudomonas stutzeri RCH2

Annotation: succinyl-diaminopimelate desuccinylase, proteobacterial clade

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR01246: succinyl-diaminopimelate desuccinylase" amino acids 10 to 379 (370 residues), 543.4 bits, see alignment E=1.3e-167 PF01546: Peptidase_M20" amino acids 67 to 376 (310 residues), 148.7 bits, see alignment E=2.2e-47 PF07687: M20_dimer" amino acids 181 to 288 (108 residues), 103.1 bits, see alignment E=8.1e-34

Best Hits

Swiss-Prot: 97% identical to DAPE_PSEU5: Succinyl-diaminopimelate desuccinylase (dapE) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01439, succinyl-diaminopimelate desuccinylase [EC: 3.5.1.18] (inferred from 97% identity to psa:PST_2690)

MetaCyc: 62% identical to succinyl-diaminopimelate desuccinylase (Escherichia coli K-12 substr. MG1655)
Succinyl-diaminopimelate desuccinylase. [EC: 3.5.1.18]

Predicted SEED Role

"N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 3.5.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHF5 at UniProt or InterPro

Protein Sequence (382 amino acids)

>Psest_1618 succinyl-diaminopimelate desuccinylase, proteobacterial clade (Pseudomonas stutzeri RCH2)
MTAPALSPTLELACELINRPSVTPLDEGCQQLMTQRLAACGFAIEPMHIEDVENFWAIRG
SEGPVLCFAGHTDVVPTGPLQAWQNPPFSARIDEQGMLHGRGAADMKGSLAAMVVAVERF
TAAHPDHKGQIAFLITSDEEGPAHHGTKAVVERLRERGQRLDWCIVGEPSSTTLVGDVVK
NGRRGSLGGTLTVRGQQGHVAYPHLAKNPIHLAAPALAELAAEHWDDGNAFFPPTSFQIS
NLNAGTGTTNVIPGTLEAVFNFRFSTESTVEQLKQRTAAILDKHGLDWNVEWALSGLPFL
TEPGALLDGVAKAIRSVTGRETTPSTSGGTSDGRFIATLGTQVVELGPVNATIHQVDEHI
LASDLDLLTDIYYLTLVNLLAC