Protein Info for Psest_1596 in Pseudomonas stutzeri RCH2

Annotation: ATPase, P-type (transporting), HAD superfamily, subfamily IC/heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 839 transmembrane" amino acids 178 to 198 (21 residues), see Phobius details amino acids 204 to 222 (19 residues), see Phobius details amino acids 241 to 259 (19 residues), see Phobius details amino acids 265 to 282 (18 residues), see Phobius details amino acids 420 to 438 (19 residues), see Phobius details amino acids 462 to 485 (24 residues), see Phobius details amino acids 777 to 798 (22 residues), see Phobius details amino acids 803 to 823 (21 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 13 to 75 (63 residues), 36.7 bits, see alignment 9.8e-13 amino acids 86 to 150 (65 residues), 39.6 bits, see alignment 1.2e-13 PF00403: HMA" amino acids 14 to 74 (61 residues), 44.1 bits, see alignment 5.5e-15 amino acids 88 to 148 (61 residues), 40.6 bits, see alignment 7e-14 TIGR01525: heavy metal translocating P-type ATPase" amino acids 239 to 823 (585 residues), 605.7 bits, see alignment E=2.5e-185 TIGR01494: HAD ATPase, P-type, family IC" amino acids 272 to 801 (530 residues), 244.5 bits, see alignment E=4.2e-76 PF00122: E1-E2_ATPase" amino acids 302 to 499 (198 residues), 180.4 bits, see alignment E=6.2e-57 PF00702: Hydrolase" amino acids 518 to 735 (218 residues), 130.2 bits, see alignment E=3.3e-41 PF12710: HAD" amino acids 651 to 732 (82 residues), 28 bits, see alignment E=7.3e-10 PF08282: Hydrolase_3" amino acids 714 to 766 (53 residues), 21.5 bits, see alignment 4.3e-08

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 72% identity to tgr:Tgr7_2514)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJH2 at UniProt or InterPro

Protein Sequence (839 amino acids)

>Psest_1596 ATPase, P-type (transporting), HAD superfamily, subfamily IC/heavy metal translocating P-type ATPase (Pseudomonas stutzeri RCH2)
MQPDQIVAFDVAELHVPGMGSDHCAGIIATSLRRVPGVSDVHANIASHKVRVEYDPRLTE
VHIVRAAVEAAGYTVAGSNAGLGRTINLSVPGMGSEHCAAIIRSTLEKLEGVAEVKASVP
AHRVSVSVGADGPSAADLKAAVEGAGYEVARVDTAQQSDDAADAVVEEAYLSQARRRLWI
AGVPTTLIMLMMAPHMFWQPIPGYLALVALMAFPVVFLYGGAATHKAAWRALCNRTANMD
VLISMGSLPPYLIGLLGFLHPMTSFVEMSATIMTFHLLGRYLEARAKGRASQAIRKLVTL
GAKTARIERDGAEIEVSVKALQPGDIMIVRPGDKIPTDGEVVGGDSHVDESIATGESLPV
YKEPGSTVLGATINKEGRLRVRATRVGGDTFLSQVIRLVDQAQGSRVPIQEFADRMTGRF
VPLVLLVSVASLLAWLLLPELLRPILLWGAGFLPWVDPTASVPVLAMLSAVAVLVIACPC
ALGLATPTALMVGSGLGAERGILIRSGEAIQTFKDVTVMVLDKTGTITRGEPRLTEVITA
AGTDEDQLLKWAASVESASEHPIARAIVEGASARGVEPDEVHEFRSAGARGVHGEVAGVP
IIIGNRLLLEEEGVTGLDALDSALDQLESRGRTAVIVGAAGVACGIVAVADTIKEESRQA
IRAMHALGLRVVMVTGDNERAARAVADEVGIDEVRAGVLPAGKVDAVRDLQARHGEHVAM
VGDGINDAPALKQANVGIAIGAGAEVAIEAADVTLVRGELTGVVEAMYLSRLTFRKIVQN
LIWASAYNGAAIPIAAFGLLHPMIGVVAMTASSLSVIGNSLLLKRRFQVALHGMRGAGV