Protein Info for HP15_1494 in Marinobacter adhaerens HP15

Annotation: histidyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 TIGR00442: histidine--tRNA ligase" amino acids 5 to 407 (403 residues), 511 bits, see alignment E=1.2e-157 PF13393: tRNA-synt_His" amino acids 9 to 311 (303 residues), 149.3 bits, see alignment E=2.4e-47 PF00587: tRNA-synt_2b" amino acids 67 to 315 (249 residues), 94.5 bits, see alignment E=1.3e-30 PF03129: HGTP_anticodon" amino acids 330 to 421 (92 residues), 29.3 bits, see alignment E=1.2e-10

Best Hits

Swiss-Prot: 69% identical to SYH_CHRSD: Histidine--tRNA ligase (hisS) from Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 85% identity to maq:Maqu_1128)

MetaCyc: 65% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PKG1 at UniProt or InterPro

Protein Sequence (426 amino acids)

>HP15_1494 histidyl-tRNA synthetase (Marinobacter adhaerens HP15)
MAKIQAIRGMNDILPEQTPVWQFVESTVRRVLAQYGYQEIRMPIVEQTDLFKRSIGEVTD
IVEKEMYTFEDRNGDSLTLRPEGTAGCVRAAEEHGLLFNQTRRLWYTGPMFRHERPQKGR
YRQFHQIGVECFGMNGPDIDAELLILTARLWKELGLAEHTRLEINSIGTSESRRVYRSAL
VDYLGQFKADLDADSQRRLESNPLRILDSKDPSTRKILENAPSLDDYLDEESREHFERLK
ALLEAAGVNYSVNPALVRGLDYYGKTVFEWITDSLGAQGTVCAGGRYDGLVEQLGGKPTF
AVGFAMGLERLILLLETLELVPEHVNSNADVYVTAMGEGALAPALVLAEQLRAALPERVI
VSHCGGGSFKSQMKKADRSGARYAVILGENEVANGTAGLKPLRADEPQAEIAQDKLADTL
AAALAG