Protein Info for Psest_1549 in Pseudomonas stutzeri RCH2

Annotation: Lysyl-tRNA synthetase (class II)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 TIGR00499: lysine--tRNA ligase" amino acids 16 to 499 (484 residues), 677.1 bits, see alignment E=6.9e-208 PF01336: tRNA_anti-codon" amino acids 68 to 141 (74 residues), 43 bits, see alignment E=5.4e-15 PF00152: tRNA-synt_2" amino acids 158 to 496 (339 residues), 315.7 bits, see alignment E=4.5e-98

Best Hits

Swiss-Prot: 88% identical to SYK_PSEF5: Lysine--tRNA ligase (lysS) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 89% identity to pfv:Psefu_1158)

MetaCyc: 62% identical to lysine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL99 at UniProt or InterPro

Protein Sequence (500 amino acids)

>Psest_1549 Lysyl-tRNA synthetase (class II) (Pseudomonas stutzeri RCH2)
MSEQPLNQHAIQEEENKLIAQRKEKLAAVREQGNAFPNDFRRDSYAGDLQKQYAEKTKEE
LEAAAIPVKVAGRIMLNRGPFMVLQDMSGRIQVYVDRKTLPAEQVEAVKHFDLGDIIAAE
GTLARSGKGDLYVYMNSVRLLTKSLRPLPDKFHGLTDTEQRYRQRYVDLMVNEEVRETFR
VRSQVISHIRKFLAERHFLEVETPMLQTIPGGAAAKPFETHHNALDMPMFLRIAPELYLK
RLVVGGFERVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQAV
LGSTDVPYGDKVFHFGEPFARLSVFDSILKYNPEISADDLNDVEKARAIAKKAGAKLLGH
EGLGKLQVMIFEELVEHKLEQPHFITEYPFEVSPLARRNDRNPNVTDRFELFIGGREIAN
AYSELNDAEDQAERFHAQVADKDAGDDEAMHFDADFVRALEYGMPPTAGEGIGIDRLVML
LTNSPSIRDVILFPHMRPEV